Characterization of the Second Form of NADH-Cytochrome b_5 Reductase Gene from Arachidonic Acid-Producing Fungus Mortierella alpina 1S-4
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概要
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The second type of cytochrome b_5 reductase (Cb5R-II) gene was characterized in the arachidonic acidproducing fungus Mortierella alpina 1S-4. Its cDNA (897 bp) and predicted amino acid (298 aa) sequences show more than 70% similarity to the previously isolated first type of Cb5R. Highly conserved exon-intron organization suggests that the two genes evolved from the duplication of a common ancestral gene. Cb5R-II has a flavin-binding domain at its highly hydrophobic N-terminal and an NADH-binding domain at the C-terminal. In comparison with Cb5R genes from other sources, high homology (46-54%) was found for yeast and plant genes. Phylogenetic analysis revealed that microbial and plant Cb5R genes represent a gene family evolved from one prototype and are different from mammalian Cb5R genes.
- 社団法人日本生物工学会の論文
- 1999-12-25
著者
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Sakuradani Eiji
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Kobayashi Miki
Biochemicals Laboratory Mitsubishi Chemical Corporation
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Sakuradani Eiji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sakuradani E
Kyoto Univ. Kyoto Jpn
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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CERTIK MILAN
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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KOBAYASHI MICHIHIKO
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Sakuradani Eiji
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Kobayashi Masatomo
Inst. Phys. Chem. Res. (riken)
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Kobayashi M
Graduate School Of Pharmaceutical Sciences Osaka University
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Kobayashi M
Carbohydrate Science Laboratory National Food Research Institute Ministry Of Agriculture Forestry An
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Certik Milan
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kobayashi Michihiko
Division Of Applied Biochemistry University Of Tsukuba
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