Breeding of a Cyclic Imide-Assimilating Bacterium, Pseudomonas putida s52, for High Efficiency Production of Pyruvate
スポンサーリンク
概要
- 論文の詳細を見る
A succinimide-assimilating bacterium, Pseudomonas putida s52, was found to be a potent producer of pyruvate from fumarate. Using washed cells from P. putida s52 as catalyst, 400 mM pyruvate was produced from 500 mM fumarate in a 36-h reaction. Bromopyruvate, a malic enzyme inhibitor, was used for the selection of mutants with higher pyruvate productivity. A bromopyruvate-resistant mutant, P. putida 15160, was found to be an effective catalyst for pyruvate production. Moreover, under batch bioreactor conditions, 767 mM of pyruvate was successfully produced from 1,000 mM fumarate in a 72-h reaction with washed cells from P. putida 15160 as catalyst.
著者
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Soong Chee-leong
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Ogawa Jun
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Horinouchi Nobuyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Soong Chee-Leong
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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HIBI Makoto
Industrial Microbiology, Graduate School of Agriculture, Kyoto University
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YOKOZEKI Kenzo
Industrial Microbiology, Graduate School of Agriculture, Kyoto University
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HAGISHITA Tairo
Industrial Microbiology, Graduate School of Agriculture, Kyoto University
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ITO Masashi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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TU Weihao
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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SEGAWA Toshinori
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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MU Xiaoqing
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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