Purification and Characterization of a Novel Alcohol Oxidase from Paenibacillus sp. AIU 311(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
An oxidase catalyzing the conversion of glycolaldehyde to glyoxal was purified to the homogeneous state from Paenibacillus sp. AIU 311, and its properties were revealed. This enzyme was specific to glycolaldehyde and glyceraldehyde, and the reaction rates to other alcohols and aldehydes were less than 6% of that of glycolaldehyde. The K_m values for glycolaldehyde and glyceraldehyde were estimated to be 13.2 and 7.5 mM, respectively. The glycolaldehyde oxidation was optimum at pH 6.5 and 50℃. The molecular mass of this enzyme was 49 kDa, and it consisted of two identical subunits of 24 kDa. The NH_2-terminal sequence was not homologous to those of alcohol oxidases. This is the first report of an oxidase exhibiting high specificity to a hydroxy group of aldehyde alcohols.
- 2007-08-25
著者
-
Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
Sasaki Yasutaka
The United Graduate School of Agricultural Sciences, Iwate University
-
Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
Ogawa Jun
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
Iwasaki Akira
Life Science R & D Center, Kaneka Co.
-
Hasegawa Junzo
Life Science R & D Center, Kaneka Co.
-
Isobe Kimiyasu
The United Graduate School of Agricultural Sciences, Iwate University
-
清水 昌
京都大学大学院農学研究科
-
Isobe Kimiyasu
Department of Agro-bioscience, Faculty of Agriculture, Iwate University
-
Kataoka M
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Kataoka Michihiko
Div. Of Applied Life Sci. Graduate School Of Agriculture Kyoto Univ.
-
Kataoka Michihiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Kato Ayako
Department of Agro-bioscience, Iwate University
-
Isobe Kimiyasu
The United Graduate School Of Agricultural Sciences Iwate University
-
Isobe Kimiyasu
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
-
Hasegawa J
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
-
Hasegawa J
Life Science R & D Center Kaneka Co.
-
Sasaki Yasutaka
Department Of Oncological Pathology Cancer Center Nara Medical University
-
Sasaki Yasutaka
The United Graduate School Of Agricultural Sciences Iwate University
-
Suzuki Shuuya
Department of Agro-bioscience, Iwate University
-
Suzuki Shuuya
Department Of Agro-bioscience Iwate University
-
Shimazu S
Div. Appl. Life Sci. Grad. Sch. Agric. Kyoto Univ.
-
Shimizu S
Agricultural Sciences Kyoto University
-
Shimizu Sakayu
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
-
Kato Ayako
Department Of Agro-bioscience Iwate University
-
Hasegawa J
Ichimaru Pharcos Co. Ltd. Gifu Jpn
-
Hasegawa Junzo
Life Science Rd Center Kaneka Corporation
-
Iwasaki Akira
Life Science R & D Center Kaneka Co.
-
Ogawa Jun
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Shiozaki Shozo
Department Of Agricultural Chemistry Kyoto University:(present Office)life Science Institute Nippon
-
Kataoka Michihiko
Divisin Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefecture
-
Hasegawa J
Fine Chemicals Research Laboratories Kaneka Corporation
-
Hasegawa Junzo
Life Science R & D Center Kaneka Co.
-
清水 晶
京都大学 大学院
-
Shimizu S
Iwate Univ. Iwate Jpn
-
清水 昌
京都大学応用生命科学専攻
-
Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
関連論文
- 1Hp23 ラッカーゼの酵母細胞表層提示による新規リグニン分解・前処理系の構築(バイオマス・資源・エネルギー工学,一般講演)
- Acidolysis and Glyceride Synthesis Reactions Using Fatty Acids with Two Pseudomonas Lipases Having Different Substrate Specificities(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 乳酸菌による共役脂肪酸生産
- ドコサヘキサエン酸高蓄積性ラビリンチュラ類の分離と栄養強化飼料としての利用
- 2Ha01 リグニンのバイオリファイナリーをめざしたラッカーゼアイソザイムの酵母細胞表層提示(バイオマス・資源・エネルギー工学,一般講演)
- Cloning, sequencing and expression analysis of a gene encoding alcohol oxidase in Paenibacillus sp. AIU 311(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Alkaline pH enhances farnesol production by Saccharomyces cerevisiae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Production and characterization of alcohol oxidase from Penicillium purpurescens AIU 063(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification and Characterization of a New Aldehyde Oxidase from Pseudomonas sp. AIU 362(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Superoxide Dismutases Exhibit Oxidase Activity on Aldehyde Alcohols Similar to Alcohol Oxidase from Paenibacillus sp. AIU 311(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Analysis of Selective Production of N^α-Benzyloxycarbonyl-L-Aminoadipate-δ-Semialdehyde and N^α-Benzyloxycarbonyl-L-Aminoadipic Acid by Rhodococcus sp. AIU Z-35-1(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification and Characterization of a Dehydrogenase Catalyzing Conversion of N^α-Benzyloxycarbonyl-L-Aminoadipic-δ-Semialdehyde to N^α-Benzyloxycarbonyl-L-Aminoadipic Acid from Rhodococcus sp. AIU Z-35-1(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNO
- Purification and Characterization of a Novel Alcohol Oxidase from Paenibacillus sp. AIU 311(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- A New Microbial Method for More Efficient Production of N^α-Benzyloxycarbonyl-L-Aminoadipate δ-Semialdehyde and N^α-Benzyloxycarbonyl-D-Aminoadipate δ-Semialdehyde(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- セサミンとの遭遇(ビタミン・バイオファクターの新展開 : 健康と食)
- 3S2a05 油糧微生物の代謝工学と機能性脂質生産への利用(発酵研究のフロンティア,シンポジウム)
- 3S7p05 プレニルアルコールの微生物生産(脂質工学の将来像,シンポジウム)
- 3S7p03 油糧微生物による有用脂質生産と特異な脂質変換反応の探索(脂質工学の将来像,シンポジウム)
- 13.油糧微生物Mortierella alpina 1S-4にみられるユニークなステロールとその生合成に関与するSterolΔ7 reductase遺伝子について(第414回研究協議会研究発表要旨,ビタミンB研究委員会)
- 2Ea07 油糧糸状菌Mortierella alpina 1S-4由来sterol methyl transferaseホモログ遺伝子のクローニングと機能解析(発酵生理学,発酵工学,一般講演)
- 2Ea08 糸状菌Mortierella alpinaからのステロール高生産変異株探索(発酵生理学,発酵工学,一般講演)
- 3D11-3 酵母様微細藻プロトテカによる炭化水素化合物の代謝経路解明(発酵生理学・発酵工学,一般講演)
- 3D11-4 Penicillium属糸状菌に特徴的なn-alkane資化経路の解明(発酵生理学・発酵工学,一般講演)
- Different Specificity of Two Types of Pseudomonas Lipases for C20 Fatty Acids with a Δ5 Unsaturated Double Bond and Their Application for Selective Concentration of Fatty Acids (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 油糧微生物の探索・開発・育種と機能性脂質生産への利用
- 1-IV-4 炭素二重結合を不斉水素化する2つの新規な旧黄色酵素(一般研究発表,新世紀ビタミン学の展望と先進的展開を目指して,日本ビタミン学会第60回大会発表要旨)
- キヌクリジノン還元酵素の機能改変を目指した構造解析
- 1-II-11 Rhodotorula rubra由来NADPH依存性3-キヌクリジノン還元酵素(一般研究発表,日本ビタミン学会第61回大会研究発表要旨)
- キラルテクノロジーにおける進化分子工学的手法の利用(生体触媒の進化分子工学と高度利用)
- 1.古くて新しい酵素「旧黄色酵素」 : 新規触媒機能の開発と応用 : 微生物のOld Yellow Enzymeの多様性と光学活性アルコール生産への利用(第404回研究協議会研究発表要旨,ビタミンB研究委員会)
- Old Yellow Enzyme の再発見 : キラルインダストリーへの利用
- 発酵は滅びゆく産業技術?
- Microbial production of conjugated fatty acids
- Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production
- 微生物酵素の産業利用 : その最前線を探る
- Identification of mutation sites on Δ12 desaturase genes from Mortierella alpina 1S-4 mutants(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Identification of mutation sites on ω3 desaturase genes from Mortierella alpina 1S-4 mutants(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Various Oils and Detergents Enhance the Microbial Production of Farnesol and Related Prenyl Alcohols(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- まえがき
- 2Ep04 高尿酸血症予防に有効なプリン塩基代謝活性を有する微生物の探索(発酵生理学,発酵工学,一般講演)
- Identification of Mutation Sites on Δ6 Desaturase Genes from Mortierella alpina 1S-4 Mutants
- 2P-1069 微生物還元酵素を用いる4-ヒドロキシイソロイシン立体異性体の生産 : 異性体分析法の確立と活性菌体の評価(2a酸素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
- 2P-1068 微生物由来新規アミノ酸水酸化酵素の基質特異性解析及び生成物の同定(2a酸素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
- 2A09-4 Unique property of levodione reductase overexpressed in Escherichia coli cells : Inversion of stereospecificity in asymmetric reduction reaction
- Old Yellow Enzyme from Candida macedoniensis Catalyzes the Stereospecific Reduction of the C=C Bond of Ketoisophorone(Microbiology & Fermentation Technology)
- 2'-デオキシリボヌクレオシドおよびDNAの工業生産 : 微生物のヌクレオシド分解代謝の逆反応を利用した効率的なプロセスに期待
- 2'-デオキシリボヌクレオシドの微生物生産
- Isolation and Primary Structural Analysis of Two Conjugated Polyketone Reductases from Candida parapsilosis(Biochemistry & Molecular Biology)
- Moecular Cloning and Overexprssion of the Gene Encoding an NADPH-Dependent Carbonyl Reducatase from Candida magnoliae, Involved in Stereoselective Reduction of Ethyl 4-Chloro-3-oxobutanoate
- Diversity of 4-Chloroacetoacetate Ethyl Ester-Reducing Enzymes in Yeasts and Their Application to Chiral Alcohol Synthesis
- Occurrence of Multiple Ethyl 4-Chloro-3-Oxobutanoate-Reducing Enzymes in Candida magnoliae
- Distribution of Threonine Aldolase Activity with Different Stereospecificities in Aerobic Bacteria
- Purification and Characterization of NADPH-Dependent Carbonyl Reductase, Involved in Stereoselective Reduction of Ethyl 4-Chloro-3-oxobutanoate, from Candida magnoliae
- Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System
- The characteristics of desaturation of Trichoderma sp. AM076 and formation of 9,12,15-hexadecatrienoic acid (16:3ω1) through Δ15 desaturation of 9,12-hexadecadienoic acid
- Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid (EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a Δ12 Desaturase-defective Mutant of Mortierella alpina 1S-4(Microbiology & Fermentation Technology)
- Inhibitory Effects of Capsaicinoids on Fatty Acid Desaturation in a Rat Liver Cell Line(Food & Nutrition Science)
- C19 Odd-chain Polyunsaturated Fatty Acids (PUFAs) Are Metabolized to C21-PUFAs in a Rat Liver Cell Line, and Curcumin, Gallic Acid, and Their Related Compounds Inhibit Their Desaturation
- Formation of 8, 11, 14-Octadecatrienoic Acid (18:3 n-4) from Naturally Occurring Unique Fatty Acid, 9, 12-Hexadecadienoic Acid (16:2 n-4), in Animal Cell Cultures
- Cloning, Sequence Analysis, and Expression in Escherichia coli of the Gene Encoding Monovalent Cation-activated Levodione Reductase from Corynebacterium aquaticum M-13
- Identification of Mutation Sites on Δ5 Desaturase Genes from Mortierella alpina 1S-4 Mutants(Microbial Physiology and Biotechnology)
- Characterization of the Second Form of NADH-Cytochrome b_5 Reductase Gene from Arachidonic Acid-Producing Fungus Mortierella alpina 1S-4
- Genetic Analysis of Cytochrome b_5 from Arachidonic Acid-Producing Fungus, Mortierella alpina 1S-4 : Cloning, RNA Editing and Expression of the Gene in Escherichia coli, and Purification and Characterization of the Gene Product^1
- Linoleic Acid Isomerase in Lactobacillus plantarum AKU1009a Proved to Be a Multi-Component Enzyme System Requiring Oxidoreduction Cofactors
- Highly Active Mutants of Carbonyl Reductase S1 with Inverted Coenzyme Specificity and Production of Optically Active Alcohols
- Purification and Characterization of a Yeast Carbonyl Reductase for Synthesis of Optically Active (R)-Styrene Oxide Derivatives
- Synthesis of 4-Hydroxyisoleucine by the Aldolase–Transaminase Coupling Reaction and Basic Characterization of the Aldolase from Arthrobacter simplex AKU 626
- Microbial Production of Optically Active β-Phenylalanine through Stereoselective Degradation of Racemic β-Phenylalanine
- Development of New Bioreactor Systems and Their Applications in the Chiral Industry
- Identification and Characterization of Enzyme Catalyzing Conversion of N^α-Benzyloxycarbonyl-L-Aminoadipic-δ-Semialdehyde to N^α-Benzyloxycarbonyl-L-Aminoadipic Acid in Aspergillus niger AKU 3302(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Screening and Industrial Application of Unique Microbial Reactions Involved in Nucleic Acid and Lipid Metabolisms
- Biocatalytic Deprotection of a Cetraxate Ester by Microbacterium sp. Strain 7-1W Cells(Microbiology & Fermentation Technology)
- Changes in Composition and Content of Mycolic Acids in Glutamate-Overproducing Corynebacterium glutamicum
- NAD^+-Dependent (S)-Specific Secondary Alcohol Dehydrogenase Involved in Stereoinversion of 3-Pentyn-2-ol Catalyzed by Nocardia fusca AKU 2123
- Syntheses of Sphingomyelins and Ceramides Bearing a Docosahexaenoyl of Arachidonoyl Group
- Gene Cloning and Functional Analysis of a Second Δ6-Fatty Acid Desaturase from an Arachidonic Acid-producing Mortierella Fungus(Biochemistry & Molecular Biology)
- Glycolic Acid Production Using Ethylene Glycol-Oxidizing Microorganisms(Microbiology & Fermentation Technology)
- Purification and Characterization of a Novel Lactonohydrolase from Agrobacterium tumefaciens
- 1P012 カルボニル還元酵素S1の基質認識機構の構造学的解析(蛋白質-構造,第48回日本生物物理学会年会)
- Distribution of L-Azetidine-2-carboxylate N-Acetyltransferase in Yeast
- Efficient Production of 2-Deoxyribose 5-Phosphate from Glucose and Acetaldehyde by Coupling of the Alcoholic Fermentation System of Baker's Yeast and Deoxyriboaldolase-Expressing Escherichia coli
- Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2'-Deoxyribonucleosides(Microbiology & Fermentation Technology)
- Production of Conjugated Fatty Acids by Lactic Acid Bacteria
- Indole Hydroxylation by Bacterial Cytochrome P450 BM-3 and Modulation of Activity by Cumene Hydroperoxide
- Bilirubin Dehydrogenase, an Enzyme in Aspergillus ochraceus IB-3 Useful for Diagnostic Measurement of Bilirubin(Microbiology & Fermentation Technology)
- Structural Analysis of Conjugated Linoleic Acid Produced by Lactobacillus plantarum, and Factors Affecting Isomer Production(Microbiology & Fermentation Technology)
- Ricinoleic Acid and Castor Oil as Substrates for Conjugated Linoleic Acid Production by Washed Cells of Lactobacillus plantarum(Microbiology & Fermentation Technology)
- Purification and Characterization of the Lipase from Pseudomonas fluorescens HU380(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Screening and Development of Unique Microbial Functions and Their Industrial Applications
- Directed evolution of an aminoalcohol dehydrogenase for efficient production of double chiral aminoalcohols(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Synthesis of 4-Hydroxyisoleucine by the Aldolase-Transaminase Coupling Reaction and Basic Characterization of the Aldolase from Arthrobacter simplex AKU 626
- A Novel Lipase from Pseudomonas fluorescens HU380: Gene Cloning, Overproduction, Renaturation-Activation, Two-Step Purification, and Characterization(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli
- Purification and cDNA Cloning of LaIT2, a Novel Insecticidal Toxin from Venom of the Scorpion Liocheles australasiae
- Distribution of L-Azetidine-2-carboxylate N-Acetyltransferase in Yeast
- Linoleic Acid Isomerase in Lactobacillus plantarum AKU1009a Proved to Be a Multi-Component Enzyme System Requiring Oxidoreduction Cofactors
- Cloning and sequencing of a gene encoding of aldehyde oxidase in Pseudomonas sp. AIU 362(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification and Characterization of a New Aldehyde Oxidase from Pseudomonas sp. AIU 362
- Cloning and sequencing of a gene encoding of aldehyde oxidase in Pseudomonas sp. AIU 362
- Achlorophyllous alga Prototheca zopfii oxidizes n-alkanes with different carbon-chain lengths through a unique subterminal oxidation pathway(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)