Analysis of Selective Production of N^α-Benzyloxycarbonyl-L-Aminoadipate-δ-Semialdehyde and N^α-Benzyloxycarbonyl-L-Aminoadipic Acid by Rhodococcus sp. AIU Z-35-1(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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The factors for selective production of N^α-benzylxycarbonyl-L-aminoadipate-5-semialdehyde(N^α-Z-L-AASA) and N^α-benzyloxycarbonyl-L-aminoadipic acid (N^α-Z-L-AAA) from N^α-benzyloxy-carbonyl-L-lysine(N^α-Z-L-lysine) by Rhodococcus sp. AIU Z-35-1 were analyzed. The cultivation time was important for selective production of N^α-Z-L-AASA, since N^α-Z-L-lysine oxidizing enzyme reached maximum at the early stage of cell growth and then decreased. The differences of enzyme activities of N^α-Z-L-lysine oxidizing enzyme and N^α-Z-L-AASA dehydrogenase in pH and temperature also affected the selective production of N^α-Z-L-AASA. For efficient production of N^α-Z-L-AAA, it was important for cultivation time that N^α-Z-L-AASA dehydrogenase activity be higher than N^α-Z-L-lysine oxidizing enzyme activity, since a high concentration of N^α-Z-L-AASA inhibited N^α-Z-L-AASA dehydrogenase activity. The optimum pH of N^α-Z-L-AAA production was affected by the instability of N^α-Z-L-AASA dehydrogenase in the alkaline pH region.
2008-02-25