Cloning, sequencing and expression analysis of a gene encoding alcohol oxidase in Paenibacillus sp. AIU 311(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
We have cloned a gene encoding an alcohol oxidase (AOD) specific to aldehyde alcohols from Paenibacillus sp. AIU 311. The AOD gene contains an open reading frame consisting of 618 nucleotides corresponding to 205 amino acid residues. The deduced amino acid sequence exhibits a high similarity to that of manganese superoxide dismutases (SODs). We expressed the cloned gene as an active product in Escherichia coli BL21 cells. The productivity (total units per culture broth volume) of the recombinant AOD expressed in E. coli BL21 is 26,000-fold higher than that of AOD in Paenibacillus sp. AIU 311. The recombinant AOD also exhibits aldehyde alcohol oxidase activity and SOD activity. The recombinant cells described in this study have utility for the production of glyoxal from glycolaldehyde.
著者
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Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Sasaki Yasutaka
The United Graduate School of Agricultural Sciences, Iwate University
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Urano Nobuyuki
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Ogawa Jun
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Iwasaki Akira
Life Science R & D Center, Kaneka Co.
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Hasegawa Junzo
Life Science R & D Center, Kaneka Co.
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Isobe Kimiyasu
The United Graduate School of Agricultural Sciences, Iwate University
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清水 昌
京都大学大学院農学研究科
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清水 昌
近畿大学 農
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Kataoka M
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kataoka Michihiko
Div. Of Applied Life Sci. Graduate School Of Agriculture Kyoto Univ.
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Kataoka Michihiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Isobe Kimiyasu
The United Graduate School Of Agricultural Sciences Iwate University
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Urano Nobuyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Hasegawa J
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Hasegawa J
Life Science R & D Center Kaneka Co.
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Sasaki Yasutaka
The United Graduate School Of Agricultural Sciences Iwate University
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Shimazu S
Div. Appl. Life Sci. Grad. Sch. Agric. Kyoto Univ.
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Shimizu S
Agricultural Sciences Kyoto University
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Shimizu S
Kyoto Univ. Kyoto Jpn
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Shimizu Sakayu
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Hasegawa J
Ichimaru Pharcos Co. Ltd. Gifu Jpn
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Hasegawa Junzo
Life Science Rd Center Kaneka Corporation
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Iwasaki Akira
Life Science R & D Center Kaneka Co.
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Ogawa Jun
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Shiozaki Shozo
Department Of Agricultural Chemistry Kyoto University:(present Office)life Science Institute Nippon
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Kataoka Michihiko
Divisin Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefecture
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Hasegawa J
Fine Chemicals Research Laboratories Kaneka Corporation
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Hasegawa Junzo
Life Science R & D Center Kaneka Co.
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清水 晶
京都大学 大学院
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Shimizu Sakayu
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Shimizu S
Iwate Univ. Iwate Jpn
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清水 昌
京都大学応用生命科学専攻
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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