Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System
スポンサーリンク
概要
- 論文の詳細を見る
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP^+ and glucose, similarly to commercially available GDH.
- 社団法人日本農芸化学会の論文
- 1998-01-23
著者
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Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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WADA MASARU
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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和田 政裕
Faculty Of Pharmaceutical Sciences Josai University
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Kataoka M
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kataoka Michihiko
Div. Of Applied Life Sci. Graduate School Of Agriculture Kyoto Univ.
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Kataoka Michihiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Shimazu S
Div. Appl. Life Sci. Grad. Sch. Agric. Kyoto Univ.
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Urabe Itaru
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Urabe Itaru
Department Of Biotechnology Faculty Of Engineering Osaka University
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Yamada H
Department Of Agricultural Chemistry Facutly Of Agriculture Kyoto University
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KITA Keiko
Department of Biotechnology, Faculty of Engineering, Tottori University
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ROHANI Luh
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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YANASE Hideshi
Department of Biotechnology, Faculty of Engineering, Tottori University
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Rohani Luh
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Wada M
Osaka Univ. Osaka
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Wada M
Tokyo Medical And Dental Univ. Chiba Jpn
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Yanase Hideshi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Yamada H
Department Of Biotechnology Faculty Of Engineering Tottori University
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Kita K
Tottori Univ. Tottori Jpn
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Kita Keiko
Department Of Biotechnology Faculty Of Engineering Tottori University
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Kataoka Michihiko
Divisin Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefecture
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Yamada H
Res. Inst. Tuberculosis Tokyo Jpn
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Yamada Hideaki
Biotechnology Research Center, Toyama Prefectural University
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Kita Keiko
Department Of Food Technology Faculty Of Engineering Fukuyama University
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Wada Masaru
Department Of Bioscience Fukui Prefectural University
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Yamada H
Department Of Agricultural Chemistry Kyoto University
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Wada M
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
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Wada Masaru
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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URABE Itaru
Department of Biotechnology, Faculty of Engineering, Osaka University
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