Testicular Injury to Rats Fed on Soybean Protein-Based Vitamin B_<12>-Deficient Diet Can Be Reduced by Methionine Supplementation

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We have previously reported that rats fed on a vitamin B_<12> (B_<12>)-deficient diet containing 180 g soybean protein per kg diet showed marked histologic damage in their testes. In this paper, we report the effect of B_<12>-deficiency on B_<12>-dependent methionine synthase in the rats' testes and the effect of methionine supplementation of the diet on testicular damage. Rats were fed the soybean protein-based B_<12>-deficient diet for 120 d. We confirmed that those rats were in serious B_<12>-deficiency by measuring urinary methylmalonic acid excretion and B_<12> content in tissues. Methionine synthase activity in the testis of the B_<12>-deficient rats was less than 2% of that in B_<12>-supplemented (control) rats. To complement disrupted methionine biosynthesis, methionine was supplied in the diet. A supplement of 5 g D,L-methionine per kg diet to the B_<12>-deficient diet did not affect urinary methylmalonic acid excretion of B_<12>-deficient rats. The testicular histology of rats fed the methionine supplemented B_<12>-deficient diet was almost indistinguishable from that of control rats. Thus, we conclude that the lowered testicular methionine synthase activity is the primary cause of the histologic damage due to B_<12>-deficiency and that methionine supplementation to the diet can reduce the damage. These findings would indicate the importance of the methionine synthase activity, especially for testicular function.
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Testicular Injury to Rats Fed on Soybean Protein-Based Vitamin B_<12>-Deficient Diet Can Be Reduced by Methionine Supplementation

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