Purification and Some Properties of Cobalamin-Dependent Methionine Synthase from Rat Liver
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概要
- 論文の詳細を見る
Cobalamin-dependent methionine synthase was purified from rat liver. The enzyme activity was separated into two peaks upon Mono-Q column chromatography. Peaks I and II of the enzyme, eluted in this order, were purified 18, 000- and 44, 000-fold in overall yields of 0.7 and 1.8%, respectively. Peak II methionine synthase, the major fraction, was homogeneous as judged by SDS-polyacrylamide gel electrophoresis. The enzyme was a large monomeric protein with an apparent molecular weight of 143, 000 Da. Interconversion of the enzyme between the two peaks was not observed during purification procedures. The enzyme required S adenosylmethionine and a reducing system for activity. Ap-parent Km values of the peak II enzyme for 5-methyltetrahydrofolate and homocysteine were 75 and 1.7μM, respectively.
- 財団法人 学会誌刊行センターの論文
著者
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YAMADA Kazuhiro
Faculty of Engineering, Okayama University
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WADA Masahiro
Faculty of Pharmaceutical Sciences, Josai University
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TOBIMATSU Takamasa
Faculty of Engineering, Okayama University
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MAEKAWA Akio
Faculty of Applied Bioscience, Tokyo University of Agriculture
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Kawata Tetsunori
Faculty Of Education Okayama University
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Toraya Tetsuo
Faculty Of Engineering Okayama University
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Yamada K
Faculty Of Engineering Okayama University:(present Address)department Of Biotechnology And Life Scie
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Katayama Y
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Yamada Kazuhiro
Faculty Of Engineering Hosei University
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Wada Masahiro
Faculty Of Pharmaceutical Science Josai University
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Tobimatsu Takamasa
Faculty of Applied Bioscience, Tokyo University of Agriculture
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TOBIMATSU Takamasa
Faculty of Engineering Okayama University
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MAEKAWA Akio
Faculty of Education, Okayama University
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KAWATA Tetsunori
Faculty of Agriculture, Tokyo University of Agriculiure Okayama University
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YAMADA Kazuhiro
Faculty of Engineering Okayama University
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Wada Masahiro
Faculty of Intelligence and Informatics, Konan University
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WADA Masahiro
Faculty of Education, Okayama University
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