Immunological detection and cellular localization of the phenylalanine ammonia-lyase of a hybrid aspen
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概要
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The cellular-localization pattern of promoter activities of two phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) genes, palg1 and palg2b, of a hybrid aspen, Populus kitakamiensis, was determined using the GUS reporter system. The strong activities of palg2b promoter were detected in lignified tissues such as xylem and phloem fiber cells of the aspen stem, suggesting the specific function of the PALg2b in lignin biosynthesis. Immunoelectron microscopy and sub-cellular fractionation of xylem cells showed that the PAL activity was detected in both the plastid and cytosol of the xylem ray-parenchyma cells and the cytosol in the developing xylem cells of the aspen mature stem. Our results of the biochemical characterization of xylem PAL protein suggested that the PAL localization was varied during cell differentiation of the aspen xylem to function in lignin biosynthesis.
- 日本植物細胞分子生物学会の論文
- 2006-09-01
著者
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KATAYAMA Yoshihiro
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Nanto Kazuya
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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MOROHOSHI Noriyuki
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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Kawai S
Tochigi Research Laboratories Kao Corporation
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Yamashita Kenji
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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KAWAI Shinya
Faculty of Agriculture, Tokyo University of Agriculture and Technology
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Osakabe Yuriko
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Kawai Shinya
Department Of Agricultural Chemistry The University Of Tokyo:(present Address) Department Of Applied
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Morohoshi Noriyuki
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Yamada K
Faculty Of Engineering Okayama University:(present Address)department Of Biotechnology And Life Scie
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Kajita S
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Acriculture And Tech
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Osakabe Keishi
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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KITAMURA Hiroko
Faculty of Agriculture, Tokyo University of Agriculture and Technology
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Katayama Y
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Katayama Yoshihiro
Graduate School Of Bio-applications & System Engineering Tokyo University Of Agriculture And Tec
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Kitamura Hiroko
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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Morohoshi Noriyuki
Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture & Technology
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