Molecular Cloning and Nucleotide Sequence of the Gene for an Alkaline Protease from the Alkalophilic Bacillus sp. KSM-K16
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概要
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The gene for an alkaline protease, suitable for use in both powder and liquid detergents, from the alkalophilic Bacillus sp. KSM-K16 was cloned and sequenced. Its nucleotide sequence included an open reading frame of 1,143 bp (380 amino acids) that encoded a pre-pro-peptide (111 amino acids) and a mature protein (269 amino acids, 26,723 Da). The deduced amino acid sequence of the enzyme exhibited high homology to those of alkaline proteases from alkalophilic strains of Bacillus and moderate homology to those of subtilisins reported to date. The catalytic triad (Asp32,His64 and Ser221) and the subsite sequence (Ser125-Leu126-Gly127) of subtilisin BPN' were well conserved as Asp32,His62 and Ser215 and as Ser123-Leu124-Gly125,respectively. However, the third ("wobble") positions of sense codons revealed some marked differences between our protease and subtilisins (and related alkaline proteases).
- 社団法人日本生物工学会の論文
- 1994-07-25
著者
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Hatada Y
Japan Agency For Marine‐earth Sci. And Technol. (jamstec) Yokosuka Jpn
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Hatada Yuji
Tochigi Research Laboratories Of Kao Corporation
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Hayashi Y
Research Institute For Food Science Kyoto University
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Kobayashi Tohru
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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KOBAYASHI Tohru
Tochigi Research Laboratories of Kao Corporation
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HAKAMADA Yoshihiro
Tochigi Research Laboratories of Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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HITOMI Jun
Tochigi Research Laboratories of Kao Corporation
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Kobayashi T
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec)
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Hayashi Y
Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science Kyoto Pharmaceut
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Kobayashi Tohru
Tochigi Research Laboratories Kao Corporation
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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