Purification and Properties of a Galacturonic Acid-releasing Exopolygalacturonase from a Strain of Bacillus
スポンサーリンク
概要
- 論文の詳細を見る
An exopolygalacturonase [exo-PGase ; poly (1,4-α-D-galacturonide) galacturonohydrolase, EC 3.2.1.67] was found to be extracellularly produced by Bacillus sp.strain KSM-P443. The exo-PGase was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, through sequential column chromatographies. The enzyme had a molecular weight of approximately 45,000 and an isoelectric point of pH 5.8. The N-terminal sequence was Ser-Met-Gin-Lys-Ile-Lys-Asp-Glu-Ile-Leu-Lys-Thr-Leu-Lys-Val-Pro-Val-Phe and had no sequence similarity to those of other pectinolylic enzymes reported to date. Maximum activity toward polygalacturonic acid (PGA) was observed at 60℃ and at pH 7.0 in 100 mM Tris-HCl buffer without requiring any metal ions. When the chain length of oligogalacturonic acids increased, the apparent Km for them decreased, but the kcat values increased. This is the first bacterial exo-PGase that releases exclusively mono-galacturonic acid from PGA, di-, tri-, tetra-, and penta-galacturonic acids.
- 2001-04-23
著者
-
Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
-
Hagihara H
Tochigi Research Laboratories Kao Corporation
-
Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
-
Kobayashi Shunsuke
Department Of Electronic Engineering (div. Of Engineering Sciences) Faculty Of Technology Tokyo Univ
-
KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
-
ITO Susumu
Tochigi Research laboratories, Kao Corporation
-
Hagihara Hiroshi
Tochigi Research Laboratories, Kao Corporation
-
Kobayashi Tohru
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
-
KOBAYASHI Tohru
Tochigi Research Laboratories of Kao Corporation
-
HIGAKI Norihiko
Tochigi Research Laboratories of Kao Corporation
-
YAJIMA Noriyuki
Tochigi Research Laboratories of Kao Corporation
-
SUZUMATSU Atsushi
Tochigi Research Laboratories of Kao Corporation
-
SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
-
Kawai Shuji
Tochigi Research Laboratories Kao Corporation
-
Kobayashi T
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec)
-
Kobayashi Shunsuke
Science University Of Tokyo
-
Ito S
National Industrial Research Institute
-
Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
-
Kobayashi Shun-ichi
Department Of Physics School Of Science University Of Tokyo
-
Kobayashi Shunichi
Department Of Physics Graduate School Of Science The University Of Tokyo
-
Kobayashi Tohru
Tochigi Research Laboratories Kao Corporation
-
Ito Susumu
Tochigi Research Laboratories Kao Corporation
-
Hagihara Hiroshi
Tochigi Research Laboratories Kao Corporation
-
KOBAYASHI Shun-ichi
Faculty of Engineering Science, Osaka University:(Present address)Department of Physics, University of Tokyo
-
KOBAYASHI Shun-ichi
Department of Physics, University of Tokyo
-
Kobayashi Shunichi
Department of Physics, Faculty of Science, University of Tokyo
関連論文
- Mechanical Properties of Poly(L-Lactide) Films Controlled by Blending with Polyesters of Lignin-Derived Stable Metabolic Intermediate, 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Polyesters of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as Bio-based Plastics Exhibiting Strong Adhering Properties
- Spiral Spin Structure in the Commensurate Magnetic Phase of Multiferroic RMn_2O_5(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Reinvestigation of Simultaneous Magnetic and Ferroelectric Phase Transitions in YMn_2O_5(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- 2D-to-1D Modulated-to-Lock in Successive Magnetic Phase Transitions Associated with Ferroelectricity in ErMn_2O_5 (Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- Fusible, Elastic, and Biodegradable Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Hysteresis-Free Amorphous Chiral Nematic Reflective Guest-Host Liquid Crystal Displays
- Purification and Properties of a Galacturonic Acid-releasing Exopolygalacturonase from a Strain of Bacillus
- Hall Effect of CeB6 and CexLa1-xB6 (Proceedings of the International Conference on Strongly Correlated Electrons with Orbital Degrees of Freedom(ORBITAL2001))
- Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6
- Hyperproduction of Sebaceous cis-6-Hexadecenoic Acid by Esterase-Reduced Mutant of Rhodococcus sp. Strain(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Detergent Alkaline Proteases : Enzymatic Properties, Genes, and Crystal Structures
- M_r 25 000 protein, substrate for protein serine/threonine kinases, is identified as a part of xenopus laevis vitellogenin B1
- 洗剤用α-アミラーゼのタンパク質工学
- A Serpin with M_r 43,000 Is a Binding Protein of M_r 25,000 Protein, a Substrate for Protein Ser/Thr Kinase Detected in Xenopus laevis Oocytes
- Deduced Amino Acid Sequence and Possible Catalytic Residues of a Thermostable, Alkaline Cellulase from an Alkaliphilic Bacillus Strain
- A New High-alkaline and High-molecular-weight Pectate Lyase from a Bacillus Isolate : Enzymatic Properties and Cloning of the Gene for the Enzyme
- Thermostabilization by Proline Substitution in an Alkaline, Liquefying α-Amylase from Bacillus sp. Strain KSM-1378
- Cloning and Sequencing of a High-alkaline Pectate Lyase Gene from an Alkaliphilic Bacillus Isolate
- Purification and Properties of a Low-molecular-weight, High-alkaline Pectate Lyase from an Alkaliphilic Strain of Bacillus
- Hyperexpression of the Gene for a Bacillus α-Amylase in Bacillus subtilis Cells : Enzymatic Properties and Crystallization of the Recombinant Enzyme
- Amino Acid Sequence and Stereoselective Hydrolytic Reaction of an Endo-1, 4-β-glucanase from a Bacillus Strain
- NADP-Specific Glutamate Dehydrogenase from Alkaliphilic Bacillus sp. KSM-635: Purification and Enzymatic Properties
- Separation of Functional Domains for the α-1,4 and α-1,6 Hydrolytic Activities of a Bacillus Amylopullulanase by Limited Proteolysis with Papain
- Application of the Upstream Region of a Bacillus Endoglucanase Gene to High-level Expression of Foreign Genes in Bacillus subtilis
- Construction, Purification, and Properties of a Truncated Alkaline Endoglucanase from Bacillus sp. KSM-635
- An Alkaline Amylopullulanase from Alkalophilic Bacillus sp. KSM-1378; Kinetic Evidence for Two Independent Active Sites for the α-1,4 and α-1,6 Hydrolytic Reactions
- Molecular Cloning and Nucleotide Sequence of the Gene for an Alkaline Protease from the Alkalophilic Bacillus sp. KSM-K16
- Alkali-Resistant, Alkaline Endo-1,4-β-glucanase Produced by Bacillus sp. PKM-5430
- Fabrication of Dual-disks Microlasers in Thiophene/Phenylene Co-oligomers
- Nd Ion Doping Effects on the Multipolar Interactions in CeB_6(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- Appearance of the Phase IV in Ce_xLa_B_6 at x ∼ 0.8
- Molecular Properties of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as a Stable Metabolic Intermediate of Lignin Isolated by Fractional Precipitation with Na^+ Ion
- Construction of a Genetic Transformation System for a Freeze-tolerant Yeast Kluyveromyces thermotolerans
- Isolation and Sequencing of a Gene, C-ADE1, and Its Use for a Host-Vector System in Candida maltosa with Two Genetic Markers(Microbiology & Fermentation Industry)
- Radical-capturing Reaction of 5,7,3', 4'-Tetramethylquercetin with the AIBN Radical Initiator
- Antioxidative activity of tree phenolic constituents I : Radical-capturing reaction of flavon-3-ols with radical initiator
- Fabrication of a Zigzag Defect-Free Surface-Stabilized Ferroelectric Liquid Crystal Display Using Polyimide Orientation Film
- Newly Developed Polymer-Stabilized Ferroelectric Liquid Crystals: Microsized Bistable Domains and Monostable V-Shaped Switching
- Highly Accurate Process Proximity Correction Based on Empirical Model for 0.18 μm Generation and Beyond
- Development of An Accurate Optical Proximity Correction System for 1 Gbit Dynamic Random Access Memory Fabrication
- Unexpected Phase Behaviors of Poly(fumarate)s Carrying Tolane-based Mesogenic Side Chains
- Control of Phase-transition Behavior and Polymerization Capability by Methyl Addition to Dialkyl Fumarates Bearing Cyanobiphenyl Mesogens
- Tenacious Epoxy Adhesives Prepared from Lignin-derived Stable Metabolic Intermediate
- A Novel Biomass-based Polymer Prepared from Lignin-derived Stable Metabolic Intermediate by Copper(I)-catalyzed Azide-Alkyne Click Reaction
- Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
- Tetrahydrofolate-dependent vanillate and syringate O-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6
- Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenative polymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli
- Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6 : interaction of O-demethylase(LigX)and ring fission dioxygenase(LigZ)
- Characterization of the Cα-Dehydrogenase Gene Involved in the Cleavage of β-Aryl Ether by Pseudomonas paucimobilis
- Deterioration of Superconductive Character of Y-Ba-Cu Oxides in Time Duration : Electrical Properties of Condensed Matter
- Electron Irradiation of Y-Ba-Cu Oxides : Electrical Properties of Condensed Matter
- Substrate Specificity of Regiospecific Desaturation of Aliphatic Compounds by a Mutant Rhodococcus Strain
- Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain
- HISTOCHEMICAL DISTRIBUTION OF GUANASE IN HUMAN TISSUES WITH GUANINE IN BICINE BUFFER AS SUBSTRATE
- Histochemical distribution of guanase in various human tissues
- Specific Accumulation of Polysaccharide-Linked Hydroxycinnamoyl Esters in the Cell Walls of Irregularly Shaped and Collapsed Internode Parenchyma Cells of the Dwarf Rice Mutant Fukei 71
- EN-P35 Fluorometric assay substrate for dioxin degrading microorganisms-synthesis of dioxin-analogous fluorometric assay substrate(Section VI Environmental Biotechnology)
- EN-P29 Functional analysis of dioxin-degrading microorganism using the fluorometric substrate and chlorinated dioxin(Section VI Environmental Biotechnology)
- Dynamic Response of Surface-Stabilized Ferroelectric Liquid Crystals : Effect of Alignment Films
- Hybrid aspen with a transgene for fungal manganese peroxidase is a potential contributor to phytoremediation of the environment contaminated with bisphenol A
- Immunological detection and cellular localization of the phenylalanine ammonia-lyase of a hybrid aspen
- Molecular cloning of the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene that contributes to the construction of a new transformation system in Coriolus versicolor
- Isolation and Analysis of Cinnamic Acid 4-Hydroxylase Homologous Genes from a Hybrid Aspen, Populus kitakamiensis
- REGULATION AND EXPRESSION OF PHENYLALANINE AMMONIA-LYASE FROM A WOODY PLANT
- Characteristics of 0.25 μm Ferroelectric Nonvolatile Memory with a Pb(Zr, Ti)O_3 Capacitor on a Metal/Via-Stacked Plug
- ANALYSIS OF CINNAMIC ACID 4-HYDROXYLASE GENES FROM A WOODY PLANT(Populus kitakamiensis)
- Degradation and Solubilization of ^C-, ^C-side Chain Labeled Synthetic Lignin (Dehydrogenative Polymerizate) by Laccase III of Coriolus versicolor
- Nucleotide Sequence for the Genomic DNA Encoding an Anionic Peroxidase Gene from a Hybrid Poplar, Populus kitakamiensis
- Oxazolomycin Esters, Specific Inhibitors of Plant Transformation
- Nucleotide Sequence of the Gene for an Alkaline Endoglucanase from an Alkalophilic Bacillus and Its Expression in Escherichia coli and Bacillus subtilis
- Nucleotide Sequence of the Gene That Encodes a Neopullulanase from an Alkalophilic Bacillus
- Purification and Some Properties of an Alkaline Pullulanase from Alkalophilic Bacillus sp.KSM-1876
- Alkaline Cellulases for Laundry Detergents : Production by Alkalophilic Strains of Bacillus and Some Properties of the Crude Enzymes(Microbiology & Fermentation Industry)
- Purification and Characterization of Multiple Carboxymethyl Cellulases from Bacillus sp. KSM-522(Microbiology & Fermentation Industry)
- Alkaline Cellulase for Laundry Detergents: Production by Bacillus sp. KSM-635 and Enzymatic Properties(Microbiology & Fermentation Industry)
- Inhibitory Effect of Oxazolomycin on Crown Gall Formation(Organic Chemistry)
- Neutrophilic Bacillus Strain, KSM-522, That Produces an Alkaline Carboxymethyl cellulase(Microbiology & Fermentation Industry)
- Comparative Composition of Brown Rice Lipids (Lipid Fractions) of Indica and Japonica Rices
- Analytical Simulation of Electrooptical Performance of Multidomain Twisted Nematic Liquid Crystal Displays
- Analytical Simulation of Electrooptical Performance of Armorphous Twisted Nematic Liquid Crystal Displays
- Mesogeniv Polymer Stabilized Ferroelectric Liquid Crystal Display Exhibiting Monostability with High Contrast Ratio and Grayscale Capability
- Super Twisted Nematic (STN) Liquid Crystal Displays (LCDs) Using Spiral Polymer Aligned Nematic (SPAN) Liquid Crystals (Special Issue on Liquid-Crystal Displays)
- Effect of Polymer Network Made of Liquid Crystalline Diacrylate on Characteristics of Liquid Crystal Display Device
- Enhanced Production of Extracellular Enzymes by Mutants of Bacillus That Have Acquired Resistance to Vancomycin and Ristocetin(Microbiology & Fermentation Industry)
- Occurrence of Unusual Fatty Acids in the Earthworm, Lumbricus terrestris (Food & Nutrition)
- Two-tone interaction in phase locking of cat cochlear nerve fibers
- Isofrequency planes in the brainstem auditory nuclei of the rat as revealed by the C-deoxyglucose method
- Organogels of Lignin-derived Stable Metabolic Intermediate, 2-Pyrone-4,6-dicarboxylic Acid (PDC), Bearing Cholesteryl Groups
- Research on cell division control of woody plant
- High Level Expression of XMP Aminase in Escherichia coli and Its Application for the Industrial Production of 5'-Guanylic Acid
- Construction of a Plasmid for High Level Expression of XMP Aminase in Escherichia coli(Microbiology & Fermentation Industry)
- Chemical Composition of Lipids, Especially Triacylglycerol, in Grape Seeds(Food & Nutrition)
- Isolation and Functional Analysis of the Promoter Sequence of the Cry j 1 Gene, Which Encodes a Major Allergenic Protein in the Pollen of Japanese Cedar (Cryptomeria japonica)
- Overexpression of a fungal laccase gene induces nondehiscent anthers and morphological changes in flowers of transgenic tobacco
- Click synthesis and adhesive properties of novel biomass-based polymers from lignin-derived stable metabolic intermediate
- Liquid Crystallinity and Organogelation Behavior of Lignin-Derived Metabolic Intermediate Bearing Cholesterol Groups
- Self-organized Structure Generated by Molecular Symmetry/Asymmetry Regulation
- Characterization of the Third Glutathione S-Transferase Gene Involved in Enantioselective Cleavage of the β-Aryl Ether by Sphingobium sp. Strain SYK-6
- Carbohydrate-Active Enzymes from Alkaliphiles