Application of the Upstream Region of a Bacillus Endoglucanase Gene to High-level Expression of Foreign Genes in Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
A 0.4-kb ScaI-HpaI fragment, 199bp upstream of the structural gene for alkaline endoglucanase, from the alkalophilic Bacillus sp. KSM-64, was found to be essential for the extracellular production of the enzyme by recombinant Bacillus subtilis cells. We constructed a new vector, pHSP64 (5.5 kb), using pHY300PLK and part of the 5' region of the endoglucanase that contained a possible promoter region. Using recombinant B. subtilis cells that carried this vector, very high production of two endoglucanases and of chloramphenicol acetyltransferase was done.
- 社団法人日本農芸化学会の論文
- 1995-11-23
著者
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Ozaki Katsuya
Tochigi Research Laboratories, Kao Corporation
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Kobayashi Tohru
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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KOBAYASHI Tohru
Tochigi Research Laboratories of Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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SUMITOMO Nobuyuki
Tochigi Research Laboratories of Kao Corporation
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HITOMI Jun
Tochigi Research Laboratories of Kao Corporation
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KAWAMINAMI Shunro
Tochigi Research Laboratories of Kao Corporation
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Kobayashi T
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec)
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Ozaki Katsuya
Tochigi Research Laboratories Of Kao Corporation
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Sumitomo N
Tochigi Research Laboratories Of Kao Corporation
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Kobayashi Tohru
Tochigi Research Laboratories Kao Corporation
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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