Structural Analysis of an Extracellular Polysaccharide Bioflocculant of Klebsiella pneumoniae(Microbiology & Fermentation Technology)
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概要
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The glycoside composition and sequence of an extracellular polysaccharide flocculant of Klebsiella pneumoniae H12 was analyzed. GC and HPLC analysis of the acid-hydrolysate identified its constituent monosaccharides as D-Glc, D-Man, D-Gal, and D-GlcA in an approximate molar ratio of 3.9 : 1.0 : 2.3 :3.6. To analyze the glycoside sequence, the polysaccharide was partially hydrolyzed by acid and enzyme treatment. GC, HPLC, TLC, MALDI-TOF/MS, and 1H- and 13C- NMR spectroscopy characterized the obtained oligosaccharides. The results clarified the partial structure of H12 polysaccharide as a linear polymer of a unit of pentasaccharide with a side chain of one D-GlcA to D-Glc moiety (see below). Although the existence of other sequences or other constituent glycosides could not be fully excluded, H12 polysaccharide must be a novel types as such a complicated unit for a polymer has not so far been reported. The partial structure of a H12 polysaccharide flocculant is also discussed in this report. [chemical formula]
- 2002-07-23
著者
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KOBAYASHI Takayoshi
Department of Applied Physics and Chemistry, Institute for Laser Science, The University of Electro-
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Kobayashi Tohru
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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Kobayashi T
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec)
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Nakata K
Central Research Laboratories Mercian Corporation
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Nakata Kuniho
Japan Bioindustry Association
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Yamaguchi Takayuki
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University Engineering Department
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Yamaguchi Taichi
Laboratory Of Biophysical Chemistry College Of Agriculture Osaka Prefecture University
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Yamaguchi T
Department Biochemistry National Institute Of Agrobiological Science
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Nakata K
Japan Bioindustry Association
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TAKIGUCHI Yasuyuki
Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
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YAZAWA Yuuki
Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
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YAMAGUCHI Tatsuaki
Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
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KURANE Ryuichiro
Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
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Yazawa Yuuki
Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology
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Kobayashi Takayoshi
Department Of Applied Physics And Chemistry And Institute For Laser Science University Of Electro-co
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Takiguchi Yasuyuki
Department Of Industrial Chemistry Faculty Of Engineering Seikei University
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Kurane Ryuichiro
Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology:(present Address)biotechnology Research Center Kubota Corporation
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Kurane Ryuichiro
Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University
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