Physiological Study of Cysteine Synthase Isozymes in Spinacia oleracea Seedlings

概要

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A polyclonal antiserum against cysteine synthase (CSase, EC 4.2.99.8) isozymes (CSases 1, 2, and 3) was prepared for physiological study of the isozymes. From the results of immunotitration and double immunodiffusion analyses, the polyclonal antiserum was proved to have the same degree of reactivity toward each isozyme. The accumulation of the isozymes during germination of spinach seeds was investigated by Western blot analysis. Although CSase 1 was not detected in the cotyledons of dark-grown seedlings, it was found in the green cotyledons of seedlings that had been grown in the light. In the extract of roots, CSase 1 was not detected in any developmental stage. On the other hand, CSases 2 and 3 were detected in all developmental stages and tissues. These results indicate that the expression of CSase 1 is regulated by light, and that the regulatory mechanism of CSase 1 expression differs from those of CSase 2 and 3 expression. Western blot analysis showed that the hydrated seeds contained a new CSase band, which was different from known CSases in terms of electrophoretic mobility.
社団法人日本農芸化学会の論文
1996-10-23