Purification and Some Properties of Plant Serine Acetyltransferase

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概要

• 論文の詳細を見る

• Serine acetyltransferase (SATase) that catalyzes the conversion of L-serine to O-acetyl-L-serine (OAS) in the presence of acetyl-CoA was highly purified from rape leaf extract, using a coupled assay system in which OAS is converted to cysteine by enzymatic action of exogenous cysteine synthase. Through purification procedures including heat treatment, ammonium sulfate fractionation and successive column chromatographies on DEAE-Toypearl, Blue-Toyopearl and Toyopearl HW-55, the specific activity was raised to about 4,800-fold over that in the crude extract. The molecular weight of rape enzyme was estimated to be about 350,000 by gel filtration column chromatography. The cysteine-forming activity of the final preparation was completely dependent on L-serine, acetyl-CoA and sulfide. However, this preparation had low activity for L-cysteine synthesis from L-serine even in the absence of exogenous cysteine synthase, suggesting that plant SATase exists as a high-molecular weight enzyme complexed with cysteine synthase.

• 日本植物生理学会の論文

著者

• Nakamura K

  Nagoya Univ. Nagoya Jpn

• Fukushima K

  Nihon Univ. School Of Dentistry At Matsudo Matsudo Jpn

• MASADA Masahiro

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• Fukushima Kazuo

  Department of Microbiology, Nihon University School of Dentistry at Matsudo

• TAMURA Goro

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Tamura Goro

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Tamura Goro

  Department Of Agricultural Chemistry Faclty Of Horticulture Chiba University

• Nakamura K

  Lab. Biochem. Grad. School Of Bioagr. Sci. Nagoya Univ.:dept. Developmental Biol. Natl. Inst. Basic

• Nakamura Katsuhito

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Hayama Atsushi

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Hayama Atsushi

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Nakamura Katsuhito

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Fukushima Kazuo

  Department Of Environmental Sciences Faculty Of Science Shinshu University

• Nakamura Kenzo

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Tamura G

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Masada M

  Chiba Univ. Chiba Jpn

• Masada Masahiro

  Department Of Biology Tokyo Metropolitan University

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