Purification and Characterization of Ferredoxin-Sulfite Reductase from Turnip (Brassica rapa) Leaves and Comparison of Properties with Ferredoxin-Sulfite Reductase from Turnip Roots

スポンサーリンク

概要

• 論文の詳細を見る

• Ferredoxin-sulfite reductase (Fd-SiR) [hydrogen-sulfide: ferredoxin oxidoreductase, EC 1.8.7.1] from turnip leaves (SiR-L) has been purified to homogeneity and its enzymatic properties compared with that from turnip roots (SiR-R). Each enzyme had a molecular mass of 64.5±0.5 kDa by SDS-PAGE and an isoelectric point of 5.15±0.05. Although each had a pH optimum around 7.8 with the same effects of inhibitors, SiR-L had higher heat stability at 60℃ than SiR-R. Moreover, SiR-R had a lower K_m and a higher specificity constant (k_<cat>/K_m) for turnip leaf ferredoxin than SiR-L. The N-terminal amino acid sequence of SiR-L was different from that of SiR-R. The results of amino acid analysis and peptide mapping suggested that SiR-L and SiR-R have different primary structures.

• 社団法人日本農芸化学会の論文
• 1997-09-23

著者

• TAKAHASHI SHUNJI

  Department of Anesthesiology, Nagasaki University School of Medicine

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• TAMURA Goro

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Tamura Goro

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Tamura Goro

  Department Of Agricultural Chemistry Faclty Of Horticulture Chiba University

• YIP Wai-Cheung

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• Yip Wai-cheung

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Takahashi Shunji

  Department Of Anesthesiology Nagasaki University School Of Medicine

• Tamura G

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

関連論文

• Quinolactacins A, B and C : Novel Quinolone Compounds from Penicillium sp. EPF-6 II. Physico-chemical Properties and Structure Elucidation
• Quinolactacins A, B and C : Novel Quinolone Compounds from Penicillium sp. EPF-6 I. Taxonomy, Production, Isolation and Biological Properties
• YM-170320, a Novel Lipopeptide Antibiotic Inducing Morphological Change of Colonies in a Mutant of Candida tropicalis pK233
• Negative inotropic action of propofol is enhanced in the acute ischemic myocardium of dogs
• Increased incidence of interstitial pneumonia by CHOP combined with rituximab
• Efficacy of S-1 in heavily pretreated patients with metastatic breast cancer : cross-resistance to capecitabine
• Does Lapatinib, a Small-Molecule Tyrosine Kinase Inhibitor, Constitute a Breakthrough in the Treatment of Breast Cancer?
• Efficacy and Safety of Combined Trastuzumab and Paclitaxel Therapy as a Second-Line Treatment in Women with Metastatic Breast Cancer : A Single Institutional Experience
• High Prevalence of HER-2/neu and p53 Overexpression in Inflammatory Breast Cancer
• Byelyankacin : A Novel Melanogenesis Inhibitor Produced by Enterobacter sp. B20
• Studies on the Biosynthesis of Terpenoid Compounds Produced by Actinomycetes 3. Biosynthesis of the Isoprenoid Side Chain of Novobiocin via the Non-mevalonate Pathaway in Streptomyces niveus
• Gene Therapy for Breast Cancer.-Review of Clinical Gene Therapy Trials for Breast Cancer and MDR1 Gene Therapy Trial in Cancer Institute Hospital
• Biosynthesis of Quinolactacin A, a TNF Production Inhibitor
• Screening of Actinomycetes with Antinematodal Properties
• Inhibitory Effect of Antimicrobial Agents on Alginate Production in Pseudomonas aeruginosa
• The Structures of Pyridovericin and Pyridomacrolidin, New Metabolites from the Entomopathogenic Fungus, Beauveria bassiana
• Pyridovericin and Pyridomacrolidin: Novel Metabolites from Entomopathogenic Fungi, Beauveria bassiana
• Melanoxazal, New Melanin Biosynthesis Inhibitor Discovered by Using the Larval Haemolymph of the Silkworm, Bombyx mori Production, Isolation, Structural Elucidation, and Biological Properties
• A New Melanin Biosynthesis Inhibitor, Melanoxadin from Fungal Metabolite by Using the Larval Haemolymph of the Silkworm, Bombyx mori
• New Method for Screening the Melanin Biosynthesis Inhibitor by Using the Larval Haemolymph of the Silkworm,Bombyx mori,and the Inhibitory Activity of Trichoviridin
• Tolerability and Safety of Classic Cyclophosphamide, Methotrexate, and Fluorouracil Treatment in Japanese Patients with Early Breast Cancer
• Production of Thermotolerant N-Carbamyl-D-Amino Acid Amidohydrolase by Recombinant Escherichia coli
• Thermostability Reinforcement through a Combination of Thermostability-related Mutations of N-Carbamyl-D-Amino Acid Amidohydrolase
• Immobilization of N-Carbamyl-D-Amino Acid Amidohydrolase
• Relationship Between an Increase in Thermostability and Amino Acid Substitutions in N-Carbamyl-D-Amino Acid Amidohydrolase
• Increase in Thermostability of N-Carbamyl-D-Amino Acid Amidohydrolase on Amino Acid Substitutions
• Screening, Characterization, and Cloning of the Gene for N-Carbamyl-D-Amino acid Amidohydrolase from Thermotolerant Soil Bacteria
• Isolation of Agrobacterium sp. Strain KNK712 That Produces N-Carbamyl-D-Amino Acid Amidohydrolase, Cloning of the Gene for this Enzyme, and Properties of the Enzyme
• Facile Syntheses of ^C-2-Butenedinitrile and Regiospecifically Labeled ^C,^N-Pyrido xines
• Kinetics of Thermostable Alanine Racemase of Bacillus stearothermophilus
• A Facile Synthesis of ε-Pyridoxinyl-L-lysines
• Adxanthromycins A and B, New Inhibitors of ICAM-1/LFA-1 Mediated Cell Adhesion Molecule from Streptomyces sp. NA-148 : II. Physico-chemical Properties and Structure Elucidation
• Detection of the Mevalonate Pathway in Streptomyces Species by Utilization of the 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Gene
• Establishment and Characterization of GSA-1, a Human Cell Line Highly Susceptible to Apoptosis after Free-fall
• A Search for Pseudomonas Alginate Biosynthesis Inhibitors from Microbial Metabolites
• Several Antifeedants from Neem Oil, Azadirachta indica A. Juss., against Reticulitermes speratus Kolbe (Isoptera : Rhinotermitidae)
• Antifeedants from Citrus natsudaidai HAYATA against Termite Reticulitermes speratus KOLBE(Pesticide Chemistry)
• Purification and Characterization of a Novel Prolyl Aminopeptidase from Maitake (Grifola frondosa)
• Effects of Nucleotides on N-Acetyl-D-Glucosamine 2-Epimerases (Renin Binding Proteins)
• Identification of a Domain Conferring Nucleotide Binding to the N-Acetyl-D-Glucosamine 2-Epimerase (Renin Binding Protein)
• Identificaion of a Domain Conferring Nucleotide Binding to the N-Acetyl-D-Glucosamine 2-Epimerase(Renin Binding Protein)
• Effects of nucleotides on N-acetyl-D-glucosamine 2-pimerases (renin-binding proteins): comparative biochemical studies
• Identification of functionally important cysteine residues of the human renin-binding protein as the enzyme N-acetyl-D-glucosamine 2-epimerase
• Effects of Nucleotides on N-Acetyl-D-Glucosamine 2-Epimerases (Renin-Binding Proteins) : Comparative Biochemical Studies
• Isolation and Characterization of Chitinase from a Flake-chitin Degrading Marine Bacterium, Aeromonas hydrophila H-2330
• Molecular Cloning of Acid-Stable Glucose Isomerase Gene from Streptomyces olivaceoviridis E-86 by a Simple Two-Step PCR Method, and Its Expression in Escherichia coli
• Identification of Functionally Important Cysteine Residues of the Haman Renin-Binding Protein as the Enzyme N-Acetyl-D-Glucosamine 2-Epimerase 1
• Renin Inhibits N-Acetyl-D-Glucosamine 2-Epimerase (Renin-Binding Protein)^1
• A Novel Metalloproteinase, Almelysin, from a Marine Bacterium, Alteromonas sp. No. 3696 : Purification and Characterization
• Substrate Specificity of a Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800
• Cloining and Expression of an Isovaleryl Pepstatin-Insensitive Carboxyl Proteinase Gene from Xanthomonas sp. T-22
• A Novel Proteinaceous Kex 2 Proteinase Inhibitor, Kexstatin, from Streptomyces platensis Q268
• A Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800: Purification and Characterization
• Effects of nucleotides on the interaction of renin with GlcNAc 2-epimerase (renin binding protein, RnBP)
• Paenidase, a novel D-aspartyl endopeptidase from paenibacillus sp. B38: purification and substrate specificity
• Refolding and Activation of Human Prorenin Expressed in Escherichia coli : Application of Recombinant Human Renin for Inhibitor Screening
• Effects of Nucleotides on the Interaction of Renin with GlcNAc 2-Epimerase (Renin Binding Protein, RnBP)
• Paenidase, a Novel D-Aspartyl Endopeptidase from Paenibacillus sp. B38 : Purification and Substrate Specificity
• Amino acid residue conferring the nucleotide binding properties of N-acetyl-D-glucosamine 2-epimerase(renin binding protein)
• Remodeling of Actin Cytoskeleton in Lupeol-Induced B16 2F2 Cell Differentiation
• Role of p38 MARK in Lupeol-Induced B16 2F2 Mouse Melanoma Cell Differentiation
• Role of p38 MAPK in Lupeol-Induced B16 2F2 Mouse Melanoma Cell Differentiation
• Differentiation- and Apoptosis-Inducing Activities by Pentacyclic Triterpenes on a MOuse Melanoma Cell Line
• Stimulative Effects of (22E,24R)-Ergosta-7,22-diene-3β,5α,6β-triol from Fruiting Bodies of Tricholoma auratum, on a Mouse Osteoblastic Cell Line, MC3T3-E1
• Human renin inhibitory activity in legumes
• Human renin inhibitory activity in legumes
• Refolding and activation of human prorenin expressed in escherichia coli: application of recombinant human renin for inhibitor screening
• Human Renin-Binding Protein Is the Enzyme N-Acetyl-D-Glucosamine-2-Epimerase
• Renin inhibitory activity in rice and cereals
• Identification of Cysteine-380 as the Essential Residue for the Human N-Acetyl-D-Glucosamine 2-Epimerase (Renin Binding Protein)
• Purification and Characterization of Ferredoxin-Sulfite Reductase from Turnip (Brassica rapa) Leaves and Comparison of Properties with Ferredoxin-Sulfite Reductase from Turnip Roots
• Effect of Sulfur and Nitrogen Nutrition on Derepression of Ferredoxin-Sulfite Reductase in Leek Seedlings
• Preparation of a Demethylallosamidin Isomer Having an N,N'-Diacetylchitobiosyl Moiety and Its Potent Inhibition against Yeast Chitinase
• Synthesis of a New Allosamidin Analog, N,N'-Diacetyl-β-Chitobiosyl Allosamizoline, and Its Inhibitory Activity against Some Chitinases
• Effect of the Quantity and Nutritional Quality of Dietary Proteins on the Transcriptional Activity of Insulin-like Growth Factor-I and Insulin-like Growth Factor Binding Protein-1 Genes
• Effects of Dexamethasone on the Production of Insulin-like Growth Factor-I and Insulin-like Growth Factor Binding Proteins in Primary Cultures of Rat Hepatocytes
• Possible use of combination chemotherapy with mitomycin C and methotrexate for metastatic breast cancer pretreated with anthracycline and taxanes
• Complete Resection After Imatinib Treatment of a Gastrointestinal Stromal Tumor of the Ileum with Peritoneal Metastases : Report of a Case
• Purification and Characterization of Ferredoxin-Sulfite Reductases from Leek(Allium tuberosum) Leaves
• Purification and Characterization of Cysteine Proteinase from a Baculovirus Gene
• Detection of the Mevalonate Pathway in Streptomyces Species Using the 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Gene
• Insulin-like Growth Factor-I Potentiates Protein Synthesis Induced by Thyrotropin in FRTL-5 Cells:Comparison of Induction of Protein Synthesis and DNA Synthesis
• Molecular Cloning and Characterization of a Broad Substrate Terpenoid Oxidoreductase from Artemisia annua
• Isolation of Human Renin Inhibitor from Soybean : Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean
• Reduction of Blood Pressure by Soybean Saponins, Renin Inhibitors from Soybean, in Spontaneously Hypertensive Rats
• Adxanthromycins A and B, New Inhibitors of ICAM-1/LFA-1 Mediated Cell Adhesion Molecule from Streptomyces sp. NA-148 : I. Taxonomy, Production, Isolation and Biological Activities
• The Occurrence of Renin Inhibitor in Rice : Isolation, Identification, and Structure-Function Relationship
• Substrate Specificity of a Novel Alcohol Resistant Metalloproteinase, Vimelysin, from vibrio sp.T1800
• Characterization of Acid-Stable Glucose Isomerase from Streptomyces sp., and Development of Single-Step Processes for High-Fructose Corn Sweetener (HFCS) Production
• Dexamethasone Stabilizes IGFBP-1 mRNA in Primary Cultures of Rat Hepatocytes
• Construction and Characterization of Escherichia coli Disruptants Defective in the yaeM Gene
• Phase I study of inotuzumab ozogamicin (CMC-544) in Japanese patients with follicular lymphoma pretreated with rituximab-based therapy
• Design and Synthesis of a Potential Endoglycosidase Inhibitor : Chemical Conversion of N, N′-Diacetylchitobiose into Novel Pseudodisaccharide Containing a Fivemembvered Cyclic N, N-Dimethylguanidine
• Starvation-increased Insulin-dependent Tyrosine Phosphorylation of the 195-kDa Protein in Intact Rat Liver
• Production of Insulin-like Growth Factors and Their Binding Proteins in Primary Cultures of Rat Liver Parenchymal and Nonparenchymal Cells
• Citronellol and Geraniol, Components of Rose Oil, Activate Peroxisome Proliferator-Activated Receptor α and γ and Suppress Cyclooxygenase-2 Expression
• Purification and Characterization of Cysteine Proteinase from a Baculovirus Gene
• Improvement in Raw Meat Texture of Cultured Eel by Feeding of Tochu Leaf Powder
• Chitinase from Autographa californica Multiple Nucleopolyhedrovirus : Rapid Purification from Sf-9 Medium and Mode of Action
• Isolation and Identification of Human Renin Inhibitor from Aralia cordata (Udo)

もっと見る 閉じる

スポンサーリンク