Isolation of Human Renin Inhibitor from Soybean : Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean

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概要

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• We found human renin inhibitory activity in soybean and isolated the active compound, soybean renin inhibitor (SRI). The physico-chemical data on the isolated SRI were identical with those of soyasaponin I. SRI showed significant inhibition against recombinant human renin, with an IC50 value of 30 μg/ml. Kinetic studies with SRI indicated partial noncompetitive inhibition, with a Ki value of 37.5 μM. On the other hand, SRI weakly inhibited pepsin, papain, and bromeline activities, but did not inhibit other proteinases, such as trypsin, kallikrein, angiotensin converting enzyme, and aminopeptidase M. Moreover, a significant (p<0.05) decrease in the systolic blood pressure of spontaneously hypertensive rats was observed when partially purified SRI was orally administrated at 40 mg/kg/d for 7 weeks. This is the first demonstration of a renin inhibitor from soybean, soyasaponin I.

• 社団法人 日本農芸化学会の論文
• 2008-12-23

著者

• Hiwatashi Kazuyuki

  秋田県農林水産技術センター総合食品研究所

• Hiwatashi Kazuyuki

  東北大学 農学研究科栄養学

• HORI Kazuyuki

  Institute for Food & Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cente

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita Jpn

• Gotoh Takeshi

  Department of Engineering in Applied Chemistry, Akita University

• Hirano Kaoru

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Takahashi S

  Kyoto Univ. Kyoto Jpn

• Takahashi S

  Department Of Biosciences Teikyo University

• Kojima Hiroaki

  R & D Company Kobayashi Pharmaceutical Co. Ltd.

• Takahashi Saori

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen

• Takahashi S

  Akita Res. Inst. Of Food And Brewing

• Takahashi Saori

  Akita Res. Inst. Of Food And Brewing

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• Takahashi Senji

  Department Of Biosciences Teikyo University

• Takahashi Shunji

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Takahashi Shunji

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• SHINBO Mamoru

  Yamada Foods Co., Ltd.

• HIWATASHI Kazuyuki

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen

• YAMADA Seihan

  Yamada Foods Co., Ltd.

• Hori K

  Kao Co. Ltd. Tochigi Jpn

• Hori K

  Akita Res. Inst. Of Food And Brewing

• Hori Kazuyuki

  The Central Research Laboratory Hitachi Ltd.

• Gotoh Takeshi

  Department Of Engineering In Applied Chemistry Akita University

• Hata Keishi

  Akita Res. Inst. Of Food And Brewing

• Kojima Hiroaki

  R&d Co. Kobayashi Pharmaceutical Co. Ltd.

• Gotoh Takeshi

  Department Of Engineering In Applied Chemistry Graduate School Of Engineering And Resource Science A

• Shinbo Mamoru

  Yamada Foods Co. Ltd.

• Yamada Seihan

  Yamada Foods Co. Ltd.

• Hata K

  Akita Research Institute Of Food And Brewing

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Kojima Hiroaki

  Kobayashi Pharmaceutical Co. Ltd. R&d Company

• Takahashi S

  Department Of Biology The University Of Tokyo

• Gotoh Takeshi

  Department Of Engineering And Applied Chemistry Graduate School Of Engineering And Resource Science

• Takahashi Satoru

  Institute Of Basic Medical Sceinces And Center For Tara

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