Kinetics of Thermostable Alanine Racemase of Bacillus stearothermophilus

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概要

• 論文の詳細を見る

• Unlabeled D- and L-alanine were racemized in deuterium oxide with an alanine racemase of Bacillus stearothermophilus at saturated concentration of substrate, and various p^2H and temperature. Samples of the solution were taken at intervals, and all alanine isomers in the samples were transformed into a mixture of diastereomeric derivatives of methyl N (-)-camphanylalaninate. Their ratio was measured on a GC-Mass, and the relative rate was calculated at the initial stage of the reaction. There was little difference in the decrease rate of the optical rotation between the enantiomers. Internal proton-transfer to the antipode was almost zero for either substrate. The α-hydrogen was abstracted 1. 2-2. 3 times faster from D-alanine than from L-alanine. D-Alanine gave an almost even mixture of deuterium labeled D- and L-alanine, while L-alanine gave a mixture of labeled D- and L-alanine at a ratio of 3 : 1. These results suggest the racemase builds two different bases in the active site. The base for D-alanine may be closer to the enzyme surface, and that for L-alanine inside.

• 社団法人日本農芸化学会の論文
• 1994-05-23

著者

• Esaki Nobuyoshi

  Institute for Chemical Research, Kyoto University

• SODA Kenji

  Institute for Chemical Research, Kyoto University

• SAWADA Seiji

  Kyoto University of Education

• Sawada S

  Kinki Univ. Higashi‐osaka Jpn

• Takahashi S

  Kyoto Univ. Kyoto Jpn

• Soda Kenji

  Institute For Chemical Research Kyoto University

• Soda Kenji

  Institute For Chemical Resarch Kyoto University

• Hasegawa Toru

  Institute For Fermentation Osaka

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• TAKAHASHI Sho

  Institute for Chemical Research, Kyoto University

• Takahashi Sho

  Institute For Chemical Research Kyoto University

• Yamamoto Yukio

  Graduate School of Human and Environmental Studies, Kyoto University

• Hasegawa Takeshi

  Isotope Laboratory, Kyoto Prefectural University of Medicine

• Tanaka Yoshinari

  Kyoto University of Education

• Hayashi Sayoko

  Kyoto University of Education

• Ryu Manami

  Kyoto University of Education

• Esaki Nobuyoshi

  Institute For Chemical Resarch Kyoto University

• Tanaka Yoshinari

  Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Technology

• Hasegawa T

  Yokohama City Univ. Yokohama Jpn

• Yamamoto Y

  Faculty Of Bio-resource Sciences Akita Prefectural University

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Yamamoto Yukio

  Graduate School Of Human And Environmental Studies Kyoto University

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