Determination by ^1H-NMR of the Stereospecificity of NAD-dependent Plant L-Histidinol Dehydrogenase for Nicotinamide C-4 Hydrogen Transfer
スポンサーリンク
概要
- 論文の詳細を見る
The hydrogen-transfer stereospecificity of cabbage histidinol dehydrogenase at the C-4 position of NAD^+ was determined by means of ^1H-NMR. A dehydrogenase reaction with enzymatically prepared [4-^2H]NAD^+ was performed. The NMR spectrum of the reaction mixture showed a peak at about 2.8 ppm, indicating the production of [(4S)-^2H]NADH, indicating that the stereospecificity of the enzyme was pro-R-specific.
- 社団法人日本農芸化学会の論文
- 1995-07-23
著者
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Esaki Nobuyoshi
Institute for Chemical Research, Kyoto University
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SODA Kenji
Institute for Chemical Research, Kyoto University
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Soda Kenji
Institute For Chemical Resarch Kyoto University
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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Ohta Daisaku
International Research Laboratories
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Ohta D
Chiba‐geigy (japan) Ltd. Hyogo Jpn
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Nagai Atsuko
International Research Laboratories, Ciba-Geigy (Japan) Ltd.
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Shimoi-Nishiyama Hiroko
International Research Laboratories, Ciba-Geigy (Japan) Ltd.
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Scheidegger Alfred
International Research Laboratories, Ciba-Geigy (Japan) Ltd.
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Shimoi-nishiyama Hiroko
International Research Laboratories Ciba-geigy (japan) Ltd.
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Nagai Atsuko
International Research Laboratories Ciba-geigy (japan) Ltd.
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Scheidegger Alfred
International Research Laboratories Ciba-geigy (japan) Ltd.
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