Increased Transglycosylation Activity of Rhodotorula glutinis Endo-β-Glucanase in Media Containing Organic Solvent(Biochemistry & Molecular Biology)

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概要

• 論文の詳細を見る

• The transglycosylation of p-nitrophenyl-β-D-cellotrioside to cellotetraose catalyzed by endo-1,4-β-glucanase (cellulase, EC 3.2.1.4) from a psychrotrophic yeast, Rhodotorula glutinis KUJ 2731, was increased by addition of a miscible organic solvent in the reaction mixture. Among various organic solvents tested, acetone was most effective. The transglycosylation activity increased with an increase in acetone concentrations, while hydrolysis activity was suppressed. The transglycosylation preferably occurred at acidic pH with the optimum pH at 2 in 10 mм Gly-HCl buffer. The optimum temperature of transglycosylation was found to be 50℃ in the presence of 40% acetone.

• 社団法人日本農芸化学会の論文
• 2001-08-23

著者

• SODA Kenji

  Department of Biotechnology, Faculty of Engineering, Kansai University

• Soda K

  Kansai Univ. Osaka Jpn

• Soda Kenji

  Department Of Biotechnology Kansai University

• Soda Kenji

  Institute For Chemical Research Kyoto University

• Soda Kenji

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Soda Kenji

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Soda Kenji

  Institute For Chemical Resarch Kyoto University

• OIKAWA Tadao

  Department of Pediatrics, National Tochigi Hospital

• Soda K

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Soda K

  Department Of Biotechnology Kansai University

• Oikawa Tadao

  Department Of Biotechnology Kansai University

• TSUKAGAWA Yasuyuki

  Department of Biotechnology, Faculty of Engineering, Kansai University

• CHINO Masashi

  Department of Biotechnology, Faculty of Engineering, Kansai University

• Chino Masashi

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Oikawa Tadao

  Department Of Biotechnology Faculty Of Engineering Kansai University

• Sato Kumi

  Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive

• Soda Kenji

  Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University

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