D-Glycerate Production from L-Tartrate by Cells of Pseudomonas sp. with High Content of L-Tartrate Decarboxylase(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
The production of D-glycerate from L-tartrate was investigated with cells of Pseudomonas sp. group Ve-2, which produce i tartrate decarboxylase. The production with intact cells had several advantages over production with the cell extract; no addition of NAD^+ is required, the substrate inhibition for the reaction is diminished, and the reaction can be done at higher temperatures than the reaction with cell extract. The culture conditions and the reaction conditions were optimized. The maximal activity was obtained when the cells were cultured at 30℃ for 24 hr in a medium containing 1.0% potassium L-tartrate, 0.05% NH_4NO_3, 0.2% yeast extract, 0.3% K_2HPO_4, 0.01% MgSO_4, H_2O, and trace elements (pH 7.5). The amount of D-glycerate produced reached 106g/1 at 45℃ after incubation for 45hr, with a molar yield of almost 100%.
- 社団法人日本農芸化学会の論文
- 1991-06-23
著者
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TANAKA Hidehiko
Laboratory of Microbiology, Institute for Chemical Research, Kyoto University
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SODA Kenji
Laboratory of Microbiology, Institute for Chemical Research, Kyoto University
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Soda Kenji
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Soda Kenji
Institute For Chemical Resarch Kyoto University
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Tanaka H
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Furuyoshi Setsuo
Department of Chemistry and Materials Technology, Kyoto Institute of Technology
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NISHIGOURI Jun
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute
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KAWABATA Nariyoshi
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute
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Soda K
Department Of Biotechnology Kansai University
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Nishigouri Jun
Department Of Chemistry And Materials Technology Faculty Of Engineering And Design Kyoto Institute O
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Furuyoshi S
Department Of Chemistry And Materials Technology Kyoto Institute Of Technology
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Furuyoshi Setsuo
Department Of Agricultural And Biological Chemistry Shinshu University
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Tanaka Hidehiko
Laboratory Of Biochemistry Kyoto College Of Pharmacy
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Kawabata N
Univ. Shiga Prefecture Shiga Jpn
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Kawabata Nariyoshi
Department Of Chemistry And Materials Technology Faculty Of Engineering And Design Kyoto Institute O
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