Enzymatic Synthesis of S-Substituted L-Cysteines with Tryptophan Synthase of Escherichia coli
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概要
- 論文の詳細を見る
The α2β2 complex of tryptophan synthase from Escherichia coli catalyzes β-replacement reactions of L-serine and its derivatives (e.g., β-chloro-L-alanine and O-methyl-DL-serine) with various alkanethiols. The products from thiobenzyl alcohol and ethanethiol were isolated to demonstrate the enzymatic synthesis of the corresponding S-substituted L-cysteines. Reactivities of various S-substituent donors were examined, and thiols such as thiobenzyl alcohol, 1-propanethiol and 1-butanethiol were found to be much more efficient substituent donors than the physiological substrate, indole. In addition, tryptophan synthase catalyzes β-replacement reactions of L-threonine with thiols to form the corresponding S-substituted β-methylcysteines, which are also produced by β-addition reactions of L-vinylglycine with thiols. These enzymatic reactions facilitate the synthesis of various sulfur-containing amino acids.
- 社団法人 日本農芸化学会の論文
著者
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Esaki N
Kyoto Univ. Kyoto Jpn
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Esaki Nobuyoshi
Institute for Chemical Research, Kyoto University
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MILES Edith
Laboratory of Biochemistry and Genetics, NIDDK, National Institutes of Health
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TANAKA Hidehiko
Institute for Chemical Research, Kyoto University
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SODA Kenji
Institute for Chemical Research, Kyoto University
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Soda Kenji
Institute For Chemical Resarch Kyoto University
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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MILES Edith
Laboratory of Biochemical Pharmacology, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Insitutes of Health
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