Inactivation of Amino Acid Racemase by S-(N-Methylthiocarbamoyl)- D, L-cysteine
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概要
- 論文の詳細を見る
S-(N-Methylthiocarbamoyl)-D- and L-cysteine (MTCC)are new irreversible inhibitors of the amino acid racemase from Pseudomonas putida. Interactions between d- or l-MTCCand purified amino acid racemase were investigated. The racemase catalyzes not only racemization reactions of d- and L-MTCCbut also their α, β-elimination reactions with concomitant inactivation. This inactivation exhibits the characteristics of suicide inhibition, i.e., pseudo-first order kinetics, irreversibility, stoichiometric labeling of active enzyme by the C3 chain derived from MTCC, and protection by substrate.
- 社団法人 日本農芸化学会の論文
著者
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Esaki N
Kyoto Univ. Kyoto Jpn
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Esaki Nobuyoshi
Institute for Chemical Research, Kyoto University
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TANAKA Hidehiko
Institute for Chemical Research, Kyoto University
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SODA Kenji
Institute for Chemical Research, Kyoto University
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KIMURA Tomio
Institute for Chemical Research, Kyoto University
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