A Novel NADH-Dependent Carbonyl Reductase from Kluyveromyces aestuarii and Comparison of NADH-Regeneration System for the Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutanoate
スポンサーリンク
概要
- 論文の詳細を見る
To compare NADH-regeneration systems for the synthesis of (S)-4-chloro-3-hydroxybutanoate (ECHB), a novel NADH-dependent carbonyl reductase (KaCR1), which reduced ethyl 4-chloroacetoacetate (ECAA) to form (S)-ECHB, was screened and purified from Kluyveromyces aestuarii and a gene encoding KaCR1 was cloned. Glucose dehydrogenase (GDH) and formate dehydrogenase (FDH) were compared as enzymes for NADH regeneration using Escherichia coli cells coexpressing each enzyme with KaCR1. E. coli cells coexpressing GDH produced 45.6 g/l of (S)-ECHB from 50 g/l of ECAA and E. coli cells coexpressing FDH, alternatively, produced only 19.0 g/l. The low productivity in the case of FDH was suggested to result from the low activity and instability of FDH.
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
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Esaki Nobuyoshi
Institute for Chemical Research, Kyoto University
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Esaki Nobuyoshi
Institute For Chemical Research Kyoto University
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Kobayashi Y
Daicel Chemical Industries Ltd. Ibaraki Jpn
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Yamamoto H
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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YAMAMOTO Hiroaki
Life Science Development Center, CPI Company, Tsukuba Research Center, Daicel Chemical Industries, L
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MITSUHASHI Kazuya
Life Science Development Center, CPI Company, Tsukuba Research Center, Daicel Chemical Industries, L
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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Yamamoto Hiroaki
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Mitsuhashi Kazuya
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Kobayashi Yoshinori
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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KIMOTO Norihiro
Life Science Development Center, CPI Company, Daicel Chemical Industries, Ltd., Tsukuba Research Cen
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MATSUYAMA Akinobu
Corporate Development Center, Daicel Chemical Industries, Ltd., Tsukuba Research Center
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Kimoto Norihiko
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Yamashita H
Mathematical Systems Inc.
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Matsuyama A
Corporate Development Center Daicel Chemical Industries Ltd. Tsukuba Research Center
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