Synthesis of Ethyl (R)-4-Chloro-3-hydroxybutanoate with Recombinant Escherichia coli Cells Expressing (S)-Specific Secondary Alcohol Dehydrogenase(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
The synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate ((R)-ECHB) from ethyl 4-chloroacetoacetate was studied using whole recombinant cells of Escherichia coli expressing a secondary alcohol dehydrogenase of Candida parapsilosis. Using 2-propanol as an energy source to regenerate NADH, the yield of (R)-ECHB reached 36.6 g/l (more than 99% ee, 95.2% conversion yield) without addition of NADH to the reaction mixture.
- 社団法人日本農芸化学会の論文
- 2002-02-23
著者
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KOBAYASHI Yoshinori
Tsukuba Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Kobayashi Y
Daicel Chemical Industries Ltd. Ibaraki Jpn
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Yamamoto H
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Yamamoto Hiroaki
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Kobayashi Yoshinori
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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MATSUYAMA Akinobu
Corporate Development Center, Daicel Chemical Industries, Ltd., Tsukuba Research Center
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YAMAMOTO Hiroaki
Tsukuba Research Center, Daicel Chemical Industries, Ltd.
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MATSUYAMA Akinobu
Tsukuba Research Center, Daicel Chemical Industries, Ltd.
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Yamashita H
Mathematical Systems Inc.
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Matsuyama A
Corporate Development Center Daicel Chemical Industries Ltd. Tsukuba Research Center
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Kobayashi Yoshinori
Tsukuba Laboratory P.c.c. Technology Inc. C/o Tsukuba Research Laboratories Kyowa Hakko Kogyo Co. Lt
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Yamamoto Hiroaki
Tsukuba Research Center Daicel Chemical Industries Ltd.
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