Purification and Properties of a Carbonyl Reductase Useful for Production of Ethyl (S)-4-Chloro-3-hydroxybutanoate from Kluyveromyces lactis(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
A novel carbonyl reductase (KLCR1) that reduced ethyl 4-chloroacetoacetate (ECAA) to synthesize ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-ECHB) was purified from Kluyveromyces lactis. KLCR1 catalyzed the NADPH-dependent reduction of ECAA enantioselectively but not the oxidation of (S)-ECHB. From partial amino acid sequences, KLCR1 was suggested to be an α subunit of fatty acid synthase (FAS) but did not have FAS activity.
- 社団法人日本農芸化学会の論文
- 2002-08-23
著者
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Kobayashi Y
Daicel Chemical Industries Ltd. Ibaraki Jpn
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Yamamoto H
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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YAMAMOTO Hiroaki
Life Science Development Center, CPI Company, Tsukuba Research Center, Daicel Chemical Industries, L
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Yamamoto Hiroaki
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Kobayashi Yoshinori
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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KIMOTO Norihiro
Life Science Development Center, CPI Company, Daicel Chemical Industries, Ltd., Tsukuba Research Cen
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MATSUYAMA Akinobu
Corporate Development Center, Daicel Chemical Industries, Ltd., Tsukuba Research Center
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KIMOTO Norihiko
Life Science Development Center, CPI Company, Daicel Chemical Industries, Ltd.
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MATSUYAMA Akinobu
Life Science Development Center, CPI Company, Daicel Chemical Industries, Ltd.
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Kimoto Norihiko
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Yamashita H
Mathematical Systems Inc.
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Matsuyama A
Corporate Development Center Daicel Chemical Industries Ltd. Tsukuba Research Center
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