Purification and Properties of a Carbonyl Reductase Involved in Stereoselective Reduction of Ethyl 4-Chloro-3-oxobutanoate from Cylindrocarpon sclerotigenum IFO 31855(Microbiology & Fermentation Technology)
スポンサーリンク
概要
- 論文の詳細を見る
A NADPH-dependent carbonyl reductase (CSCR1) was purified to homogeneity from Cylindrocarpon sclerotigenum IFO 31855. The enzyme catalyzed the stereoselective reduction of ethyl 4-chloro-3-oxobutanoate to the corresponding (S) -alcohol with a >99% enantiomer excess. The relative molecular mass of the enzyme was estimated to be 68,000 by gel filtration chromatography and 24,800 on SDS polyacrylamide gel electrophoresis. The enzyme had an extremely narrow substrate specificity and it highly reduced conjugated diketone, 2, 3-butanedion, in addition to ethyl 4-chloro-3-oxobutanoate. The enzyme activity was inhibited by HgCl_2 (100%), 5, 5'-dithiobis (2-nitrobenzoic acid) (56%), dicoumarol (42%), and CuSO_4 (46%). The N-terminal amino acid sequence of the enzyme (P-Q-G-I-P-T-A-S-R-L) showed no apparent similarity with those of other oxidoreductases.
- 社団法人日本農芸化学会の論文
- 2003-06-23
著者
-
YOSHIZAKO Fumiki
Research Institute for Advanced Science and Technology, Osaka Prefecture University
-
Nishimura Atsuhisa
Research Laboratory Ichibiki Co. Ltd.:department Of Applied Biology Faculty Of Textile Science Kyoto
-
YAMAMOTO Hiroaki
Tsukuba Research Center, Daicel Chemical Industries, Ltd.
-
Yamamoto H
Kyoto Univ. Kyoto Jpn
-
Yamashita H
Mathematical Systems Inc.
-
Numata Atsushi
Research Laboratory Ichibiki Co. Ltd.:department Of Applied Biology Faculty Of Textile Science Kyoto
-
Nishimura Atsuo
Research Institute For Advanced Science And Technology Osaka Prefecture University
-
Nishimura A
Research Institute For Advanced Science And Technology Osaka Prefecture University
-
SARATANI Yuri
Research Institute for Advanced Science and Technology, Osaka Prefecture University
-
UHEDA Eiji
Research Institute for Advanced Science and Technology, Osaka Prefecture University
-
Uheda E
Research Institute For Advanced Science And Technology Osaka Prefecture University
-
Uheda Eiji
Research Institute For Advanced Science And Technology University Of Osaka Prefecture
-
Saratani Yuri
Research Institute For Advanced Science And Technology Osaka Prefecture University
-
Yoshizako F
Research Institute For Advanced Science And Technology Osaka Prefecture University
-
Yoshizako Fumiki
Research Institute For Advanced Science And Technology University Of Osaka Prefecture
-
Yamamoto Hiroaki
Tsukuba Research Center Daicel Chemical Industries Ltd.
関連論文
- APPLICATION OF ULTRATHIN CRYO-SECTIONS TO ENZYME HISTOCHEMISTRY (Histo- and Cytochemistry in Tissue Culture)
- Histochemical Study of the Golgi Appalatus Using Ultracryo Sections.
- Application of ultrathin cryo-sections to the enzyme histochemistry
- Asymmetric Reduction of Ethyl 2-methyl 3-oxobutanoate by Fungi
- Asymmetric Reduction of Ethyl 2-methyl 3-oxobutanoate by Chlorella
- Manufacturing High Purity Maltose and Maltotetraose from Starch by a Novel and Efficient Procedure Named "Reducing End Modification Method"
- Effects of Moranoline, 4-O-α-D-Glucopyranosylmoranoline and Their N-Substituted Derivatives on Thermostability of Cyclodextrin Glycosyltransferase, Glucoamylase, and β-Amylase
- Enzymatic Synthesis of High Purity Maltotetraose Using Moranoline (1-Deoxynojirimycin)
- A Novel and Efficient Method for Enzymatic Synthesis of High Purity Maltose Using Moranoline (1-Deoxynojirimycin)
- A Novel Laccase Inhibitor, N-Hydroxyglycine, Produced by Penicillium citrinum YH-31
- Structures of the Laccase-catalyzed Oxidation Products of Hydroxy-benzoic Acids in the Presence of ABTS [2,2'-Azino-di-(3-ethylbenzothiazoline-6-sulfonic Acid)]
- A Simultaneous Assay Method for L-Glutamate and L-Pyroglutamate Contents in Soy Sauce Using a 5-Oxoprolinase (without ATP Hydrolyzing Activity)
- Isolation and Characterization of a Novel 5-Oxoprolinase (without ATP-hydrolyzing)from Alcaligenes faecalis N-38A
- Effect of α-Cyclodextrin on Thermostability of Glucoamylase(Biological Chemistry)
- A Novel NADH-Dependent Carbonyl Reductase from Kluyveromyces aestuarii and Comparison of NADH-Regeneration System for the Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutanoate
- Purification and Properties of a Carbonyl Reductase Useful for Production of Ethyl (S)-4-Chloro-3-hydroxybutanoate from Kluyveromyces lactis(Microbiology & Fermentation Technology)
- Synthesis of (R)-1,3-Butanediol by Enantioselective Oxidation Using Whole Recombinant Escherichia coli Cells Expressing (S)-Specific Secondary Alcohol Dehydrogenase(Microbiology & Fermentation Technology)
- Synthesis of Ethyl (R)-4-Chloro-3-hydroxybutanoate with Recombinant Escherichia coli Cells Expressing (S)-Specific Secondary Alcohol Dehydrogenase(Microbiology & Fermentation Technology)
- Cloning and Expression in Escherichia coli of a Gene Coding for a Secondary Alcohol Dehydrogenase from Candida parapsilosis
- Purification and Characterization of (S)-1,3-Butanediol Dehydrogenase from Candida parapsilosis
- Microvasucular architecture in two distinct gingival regions divided by an accumulation of dental calculus
- Identification of 2,3-Dihydro-γ-ionylideneethanol in Cercospora cruenta
- Metabolism of Chiral Ionylideneacetic Acids on the Abscisic Acid Biosynthetic Pathway in Cercospora
- Early Biosynthetic Pathway to Abscisic Acid in Cercospora cruenta
- MEAN WAITING TIMES OF THE ALTERNATING TRAFFIC WITH STARTING DELAYS
- Gonadotropin-Releasing Hormone Analogue Therapy for Peritoneal Inclusion Cysts after Gynecological Surgery
- Biological Characteristics of Human Uterine Endometrial Cancer Variant Cells Selected for Blood Group H Type 1 Antigen : Adhesion to Vascular Endothelial Cells
- Biological Function of Type1 Carbohydrate Antigen in Endometrial Cancer
- Purification and Properties of a Carbonyl Reductase Involved in Stereoselective Reduction of Ethyl 4-Chloro-3-oxobutanoate from Cylindrocarpon sclerotigenum IFO 31855(Microbiology & Fermentation Technology)
- Stereoselective Reduction of Ethyl 4-chloro-3-oxobutanoate by Fungi(Microbiology & Fermentation Technology)
- Unsteady Flow and Heat Transfer in a Plate-Fin and Tube Heat Exchanger
- Fluid Flow and Heat Transfer in a Two-dimensional Miter-bend : Study of Unsteady Motion by Numerical Calculations : Heat Transfer, Combustion, Power,Thermophysical Properties
- Fluid Flow and Heat Transfer in a Plate-fin and Tube Heat Exchanger : Analysis of Fluid Flow around Square Cylinder Situated between Parallel Plates
- Fluid Flow and Heat Transfer in a Plate-fin and Tube Heat Exchanger : Analysis of Heat Transfer around a Square Cylinder Situated between Parallel Plates
- Fluid Flow and Heat Transfer in a Two-dimensional Miter-bend : 1st Report, Experiments and Analyses
- Study on Three-Dimensional Flow and Heat Transfer in Miter-Bend : 3rd Report, Effects of Reynolds Number and Aspect Ratio
- Study on Three-Dimensional Flow and Heat Transfer in Miter-Bend : 2nd Report, Analysis of Heat Transfer in Laminar Region
- Study on Three-Dimensional Flow and Heat Transfer in Miter-Bend : 1st Report, Analysis of Flow in Laminar Region
- Transport Capacity of a Route Order Type Demand Bus System
- Microbial Degradiation of Aspergillic Acid by Trichoderma koningii M 102
- Juvenile Hormone Activity of Juvabione Analogues against Pyrrhocoris apterus(Hemiptera:Pyrrhocoridae)
- Rapid Shedding of Roots from Azolla filiculoides Plants in Response to Inhibitors of Respiration : ENVIRONMENTAL AND STRESS RESPONSES : STRUCTURE AND FUNCTION OF CELLS
- High Concentrations of Ammonium Ions at Low pH Decrease the Number of Cyanobionts in Apical Portions of Azolla
- Decrease in the Numbers of Cyanobionts and Heterocysts and Nitrogen-Fixing Activity in Azolla Leaves upon Transfer to Low Light Intensity
- Decrease in the Numbers of Cyanobionts and Heterocysts and Nitrogen-Fixing Activity in Azolla Leaves upon Transfer to Low Light Intensity (Plant Nutrition)
- Relationship between Nitrogen Fixation and Numbers of Cyanobionts and Heterocysts in Fifteen Azolla Strains
- Free Amino Acids and Ammonia in the Azolla-Anabaena Association Grown with N_2, NH_4^+, and NO_3^-
- Disappearance of Symbiotic Algae in the Azolla-Anabaena Association Subjected to Transitory Exposure to Ammonium
- Stimulatory Effect of Acetate and Propionate on Aspergillic Acid Formation by Aspergillus oryzae A 21
- Formation of 2,6-Dihydroxyphenylacetic Acid from Phenylacetic Acid by Trichosporon cutaneum(Microbiology & Fermentation Industry)
- Abscission of Azolla Branches Induced by Ethylene and Sodium Azide
- Heat Effects for a Single Cell of Saccharomyces cerevisiae Determined using a Classic and a New Procedure
- Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach
- Microbial Reduction of Cyclohexanone by Chlorella pyrenoidosa Chick
- Microbial Reduction of 2-Norbornanone by Chlorella
- Asymmetric Reduction of Methyl 3-Oxopentanoate by Chlorella
- Purification and Characterization of an Enzyme That Catalyzes Ring Cleavage of Aspergillic Acid, from Trichoderma koningii ATCC 76666
- Biotransformation of Cyclic β-Keto Esters by Chlorella pyrenoidosa Chick
- Identification of Algal Transformation Products from Alicyclic Ketones
- Bioconversion of Cyclohexaneacetic Acid to Monohydroxycyclohexaneacetic Acids by CHlorella pyrenoidosa Chick
- Enzymatic Degradation of Aspergillic Acid by Trichoderma koningii M102
- Identification of the Degradation Products of Aspergillic Acid by Trichoderma koningii M 102
- Metabolism of n-Alkyl-Substituted Cyclohexanes with an Odd Number of Carbon Atoms in the Side Chain by Micrococcus sp. RCO-4M
- Metabolism of n-Alkylcyclohexanes with an Even Number of Carbon Atoms in the Side Chain by Micrococcus sp. RCO-4M
- Production of Optically Active 1,2,4-Butanetriol from Corresponding Racemate by Microbial Stereoinversion(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Localization of Glutamine Synthetase Isoforms in Hair Cells of Azolla Leaves
- The Role of Papillae during the Infection Process in the Gunnera-Nostoc Symbiosis
- Absorption Spectrum of CsI:Pb^ Crystal