Purification and Characterization of an Enzyme That Catalyzes Ring Cleavage of Aspergillic Acid, from Trichoderma koningii ATCC 76666
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概要
- 論文の詳細を見る
The aspergillic acid degrading enzyme (ADE) that catalyzes the cleavage of the pyrazine ring in aspergillic acid (AA, 1-hydroxy-3-isobutyl-6-sec-butyl-2-pyrazinone) was purified to electrophoretic homogeneity from extracts of Trichoderma koningii ATCC 76666. ADE was a homodimeric protein with a molecular mass of 112 kDa, contained 1 mol of FAD per mol of subunit, and required NAD(P)H and molecular oxygen for its activity. ADE had an isoelectric point of around 5.3, and an optimum pH of 7.0-8.0. p-Chloromercuribenzoate and HgCl_2 completely inhibited ADE activity, while metal chelating reagents, α, α'-dipyridyl and o-phenanthroline, were not inhibitors. The substrate specificity among AA-related compounds was that hydroxyaspergillic acid was a poor substrate (16% of the activity for AA) and deoxyaspergillic acid did not serve as a substrate.
- 社団法人日本農芸化学会の論文
- 1997-09-23
著者
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YOSHIZAKO Fumiki
Research Institute for Advanced Science and Technology, Osaka Prefecture University
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Nishimura Atsuhisa
Research Laboratory Ichibiki Co. Ltd.:department Of Applied Biology Faculty Of Textile Science Kyoto
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Numata Atsushi
Research Laboratory Ichibiki Co. Ltd.:department Of Applied Biology Faculty Of Textile Science Kyoto
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Nishimura Atsuo
Research Institute For Advanced Science And Technology Osaka Prefecture University
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Nishimura A
Research Institute For Advanced Science And Technology Osaka Prefecture University
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Yoshizako F
Research Institute For Advanced Science And Technology Osaka Prefecture University
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Yoshizako Fumiki
Research Institute For Advanced Science And Technology University Of Osaka Prefecture
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CHUBACHI MITSUO
Research Institute for Advanced Science and Technology. Osaka Prefecture University
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Chubachi M
Research Institute For Advanced Science And Technology Osaka Prefecture University
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Chubachi Mitsuo
Research Institute for Advanced Science and Technology, Osaka Prefecture University
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