Isolation and Characterization of a Novel 5-Oxoprolinase (without ATP-hydrolyzing)from Alcaligenes faecalis N-38A
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概要
- 論文の詳細を見る
A screening test was undertaken to isolate a microorganism that produced 5-oxoprolinase (without ATP-hydrolyzing). The 5-oxoprolinase (without ATP-hydrolyzing) activity (decyclization activity toward L-pyroglutamate) was found in a cell-free extract of Alcaligenes faecalis N-38A, newly isolated from a soil sample. The enzyme was purified as a homogeneous preparation. The molecular weight of the enzyme was estimated to be 47, 000. The decyclization activity was specific for L-pyroglutamate, and independent of ATP and metal ions. The reaction was a reversible one, i. e. , cyclization reaction of L-glutamate to yield pyroglutamate was identified.
- 社団法人日本農芸化学会の論文
- 1995-10-23
著者
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Oyama H
Kyoto Inst. Technol. Kyoto Jpn
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
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Oyama Hiroshi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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NUMATA Atushi
Osaka University of Pharmaceutical Sciences
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SHIN Takashi
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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Nishimura Atsuhisa
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Ozaki Yasunori
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Nishimura Atsuhisa
Research Laboratory Ichibiki Co. Ltd.:department Of Applied Biology Faculty Of Textile Science Kyoto
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Ozaki Y
Shizuoka Univ. Shizuoka Jpn
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Shin T
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Shin Takashi
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Numata Atsushi
Research Laboratory Ichibiki Co. Ltd.:department Of Applied Biology Faculty Of Textile Science Kyoto
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Nishimura A
Research Institute For Advanced Science And Technology Osaka Prefecture University
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Takenisi Shigeyuki
Osaka Municipal Technical Research Institute
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MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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SHIN Takashi
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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