Oxidative Decarboxylations of 4-Hydroxymandelic Acid and 2-(4-Hydroxyphenyl)glycine by Laccase from Coriolus versicolor and Bilirubin Oxidases from Trachyderma tsunodae and Myrothecium verrucaria
スポンサーリンク
概要
- 論文の詳細を見る
Laccase from Coriolus versicolor and bilirubin oxidases from Trachyderma tsunodae and Myrothecium verrucaria converted 4-hydroxymandelic acid (HMA) and 2-(4-hydroxyphenyl)glycine into 4-hydroxy-benzaldehyde (HBA), which was a single product and was not converted further. The reactions were oxidative decarboxylations that were considered to be caused by the enzyme-catalyzed abstractions of hydrogen from the phenolic hydroxyl groups. The decarboxylation of HMA was used for a new colorimetric measurement of the activities of these enzymes. One unit of the enzymes was defined as the amount that catalyzed the formation of 1μmol of HBA per minute. When HMA was used for a substrate, the optimum pHs of laccase, bilirubin oxidase from T. tsunodae, and bilirubin oxidase from M. verrucaria were 4.5, 5.0, and 8.5, respectively, and their K_m values were 31.3mM, 34.5mM, and 22.3mM, respectively.
- 社団法人日本農芸化学会の論文
- 1993-11-23
著者
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Nishida Hiroshi
Department Of Cardiovascular Surgery The Heart Institute Of Japan Tokyo Women's Medical Univers
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OKAMOTO ROKURO
Central Research Laboratories, Sanraku-Ocean Co., Ltd.,
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
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SHIN Takashi
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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AGEMATU Hitosi
Central Research Laboratories, Mercian Corporation
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Shin T
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Shin Takashi
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Agematu Hitosi
Central Research Laboratories Mercian Corporation
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Okamoto R
Mercian Corp. Fujisawa Jpn
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Nishida H
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Shibamoto Norio
Central Research Laboratories, Mercian Co.
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Nishida Hiroshi
Central Research Laboratories, Mercian Co.
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Shibamoto Norio
Central Research Laboratories Mercian Co.
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Nishida H
Department Of Cardiovascular Surgery The Heart Institute Of Japan Tokyo Women's Medical Univers
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Agematu Hitosi
Central Research Laboratories Mercian Co.
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Agematu H
Mercian Corp. Fujisawa Jpn
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Okamoto Rokuro
Central Research Laboratories Mercian Corporation
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Takenisi Shigeyuki
Osaka Municipal Technical Research Institute
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Nishida Hiroshi
Central Research Laboratories Sanraku-ocean Co. Ltd.
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MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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SHIN Takashi
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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