Isolation and Characterization of a Proteinaceous Protease Inhibitor from Bacillus subtilis(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
A proteinaceous protease inhibitor which might have an intracellular role in modulating protease activity during sporulation was isolated from B. subtilis IFO 3027 by trichloroacetic acid and ethanol precipitations, and column chromatographies on SP-Sephadex, DEAE-Sephadex, DEAE-Toyopearl and Sphadex G-75. The molecular weight of the inhibitor was estimated by gel filtration and SDS polyacrylamide gel electrophoresis to be about 16,000. The isoelectric point was determined as pH 4.8. The inhibitor is an acid and thermostable protein. The amino acid sequence in the amino terminal region was determined to be (Met)-Glu-Asn-Gln-Glu-Val-Val-Leu-X-X-Asp-Ala-Ile-Gln-Glu・・・(X, unidentified). In addition to cytoplasmic serine proteases of the inhibitor-producing strain, the inhibitor inhibits various microbial serine proteases.
- 社団法人日本農芸化学会の論文
- 1986-12-23
著者
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Nishino T
Tohoku Univ. Miyagi Jpn
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SHIMIZU YASUHIRO
Department of Surgery, Aichi Cancer Center Hospital
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FUKUHARA Ken-ichi
Central Research Laboratories, Ajinomoto Co., Inc.
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Shimizu Yasuhiro
Department Of Gastroenterological Surgery Aichi Cancer Center Hospital
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Shimizu Yasuhiro
Laboratory Of Applied Biochemistry Faculty Of Textile Science Kyoto Institute Of Technology
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NISHINO Toyokazu
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Fukuhara Ken-ichi
Central Research Laboratories Ajinomoto Co.
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Nishino Toyokazu
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Shimizu Yasuhiro
Department Of Applied Physics University Of Tokyo:division Of Chemistry Kyoto University:(present Ad
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SHIMIZU Yasuhiro
Department of Agricultural Chemistry, College of Agriculture, University of Osaka prefecture
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