Dye-sensitized Photooxidation of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Histidine Residue Oxidized(Biological Chemistry)

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概要

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• The neutral protease of Bacillus subtilis var.amylosacchariticus was photooxidized in the presence of methylene blue, by which treatment the enzyme was rapidly inactivated. The inactive enzyme was digested with endoproteinase Asp-N, the resultant peptides were separated by HPLC, and their amino acid sequences were compared with those obtained from the unmodified enzyme. Of four peptides that contained histidine residues, only the recovery of one peptide was found to be decreased by the photooxidation with the appearance of a new peptide. Comparisons of amino acid compositions and sequences between these two peptides showed that the latter peptide lacked His^<228> of the former one, indicating that His^<228> was photooxidized. This result suggests that His^<228> is involved in the catalytic reaction of the neutral protease or interaction with substrates.

• 社団法人日本農芸化学会の論文
• 1991-08-23

著者

• Kanatani Akio

  School Of Pharmaceutical Sciences Nagasaki University

• YOSHIMOTO TADASHI

  School of Pharmaceutical Sciences, Nagasaki University

• TSURU DAISUKE

  School of Pharmaceutical Sciences, Nagasaki University

• Yoshimoto T

  Nagasaki Univ. Nagasaki Jpn

• Yoshlmoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasiki University

• MORIKAWA Shin-ya

  School of Pharmaceutical Sciences, Nagasaki University

• Murao S

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Murao S

  Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture

• Morikawa Shin-ya

  School Of Pharmaceutical Sciences Nagasaki University

• Murao S

  Sojo Univ. Kumamoto Jpn

• Tsuru D

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Tsuru Daisuke

  Department Of Applied Microbial Technology Sojo University

• Tsuru D

  Department Of Applied Microbial Technology Sojo University

• Tsuru Daisuke

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tomotaka

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

• Yoshimoto Tadashi

  School of Pharamaceutical Sciences, Nagasaki University

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