Purification and Some Properties of Lignostilbene-α,β-dioxygenase Responsible for the C_α-C_β Cleavage of a Diarylpropane Type Lignin Model Compound from Pseudomonas sp. TMY1009(Biological Chemistry)
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概要
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A novel dioxygenase, lignostilbene-α,β-dioxygenase (LSD), which catalyzes cleavage of the interphenyl double bond of lignin-derived stilbenes, was isolated. Four isozymes of LSD were separated from cell-free extracts of Pseudomonas sp. TMY1009 by ion-exchange chromatography on a DEAE-Toycpearl column. The major isozyme, LSD-I, was purified to electrophoretic homogeneity and characterized. LSD-I cleaved the interphenyl double bond of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene with the optimum pH at 8.5. The Km of LSD-I was 11μM for the stilbene and 110μM for oxygen. The molecular weight of LSD-I, which is composed of two identical subunits, was estimated to be 94,000. LSD-I contained 1 g atom of iron per 1 mol of enzyme protein.
- 社団法人日本農芸化学会の論文
- 1989-10-23
著者
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Habu Naoto
Department of Bioproductive Science, Faculty of Agriculture, Utsunomiya University
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Samejima Masahiro
Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The Universi
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Yoshlmoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Samejima Masahiro
Department Of Biomaterial Sciences Graduate School Of Agricultural And Life Sciences University Of T
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Habu Naoto
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Habu Naoto
Department Of Bioproductive Science Faculty Of Agriculture Utsunomiya University
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Samejima M
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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YOSHIMOTO Tomotaka
Department of Forest Products, Faculty of Agriculture, The University of Tokyo
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KAMODA Shigehiro
Department of Forest Products, Faculty of Agriculture, The University of Tokyo
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Yoshimoto Tomotaka
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Kamoda Shigehiro
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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