Complete Amino Acid Sequence of Neutral Protease from Bacillus subtilis var. amylosacchariticus(Biological Chemistry)
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概要
- 論文の詳細を見る
The neutral protease of Bacillus subtilis var. amylosacchariticus was cleaved chemically or digested with proteolytic enzymes, and the resultant peptides were separated and purified by high performance liquid chromatography. The sequence analyses of these peptides by the manual Edman procedure established the complete amino acid sequence of the enzyme. The neutral protease consisted of 300 amino acid residues with Ala and Leu as its amino- and carboxyl-termini, respectively, and the molecular weight was calculated to be 32,633. The sequence was found to be identical to that of B. subtilis IA72 neutral protease, which was deduced from nucleotide sequencing. Comparison of the sequence with those of other Bacillus proteases revealed that the putative active site amino acid residues, Zn-binding ligands, and two Ca-binding sites were well conserved among them, as compared with those of thermolysin.
- 社団法人日本農芸化学会の論文
- 1989-10-23
著者
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YOSHIMOTO TADASHI
School of Pharmaceutical Sciences, Nagasaki University
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TSURU DAISUKE
School of Pharmaceutical Sciences, Nagasaki University
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Yoshimoto T
Nagasaki Univ. Nagasaki Jpn
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Yoshlmoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasiki University
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KOBAYASHI Ryuji
School of Pharmaceutical Sciences, Nagasaki University
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Kobayashi Ryuji
School Of Pharmaceutical Sciences Nagasaki University
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Kobayashi Ryuji
School Of Health Science Kibi International University
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Tsuru D
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Tsuru Daisuke
Department Of Applied Microbial Technology Sojo University
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Tsuru D
Department Of Applied Microbial Technology Sojo University
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Tsuru Daisuke
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tomotaka
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Yoshimoto Tadashi
School of Pharamaceutical Sciences, Nagasaki University
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