Expression and Secretion of Scytalidopepsin B, an Acid Protease from Scytalidium lignicolum, in Yeast
スポンサーリンク
概要
- 論文の詳細を見る
An expression and secretion system of scytalidopepsin B, an acid protease from Scytalidium lignicolum, was constructed in yeast. Saccharomyces cerevisiae AH22 was transformed with an yeast-E. coli shuttle vector, pAM82,in which an yeast invertase signal segment and the cDNA encoding the pro-and mature enzyme regions were inserted. The transformant was found to secret a pepstatin-insensitive acid protease, when cultured aerobically in a low phosphate (Pi) medium. Amino terminal amino acid sequencing analysis indicated that the recombinant acid protease was accurately processed and secreted as a mature form.
- 社団法人日本農芸化学会の論文
- 2000-07-23
著者
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ODA Kohei
Faculty of Science and Technology, Sophia University
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Oyama H
Kyoto Inst. Technol. Kyoto Jpn
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Tsuru Daisuke
Department of Applied Microbiology, Kumamoto Institute of Technology
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SHIMUTA Ken
Department of Bacteriology, National Institute of Infectious Diseases
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Oyama Hiroshi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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ODA-UEDA Naoko
Department of Applied Microbial Technology, sojo University
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WASHIO Masahiro
Department of Applied Microbial Technology, sojo University
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OYAMA Hiroshi
Faculty of Textile Science, Kyoto Institute of Technology
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Oda Kohei
Faculty Of Science And Technology Sophia University
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Oda N
Department Of Applied Microbial Technology Sojo University
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Tsuru D
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Tsuru Daisuke
Department Of Applied Microbial Technology Sojo University
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Tsuru Daisuke
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Oda K
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Tsuru D
Department Of Applied Microbial Technology Sojo University
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Washio Masahiro
Department Of Applied Microbial Technology Sojo University
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Shimuta Ken
Department Of Applied Microbial Technology Sojo University
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Oyama Hiroshi
Faculty Of Science And Engineering University Of Setsunan
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