Purification and Characterization of Arctium lappa L. (Edible Burdock) Polyphenol Oxidase
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概要
- 論文の詳細を見る
Polyphenol oxidase of Arctium lappa L. (edible burdock) has been purified by chromatographies on DEAE-cellulose, Sephadex G-75, and phenyl-Cellulofine to a homogeneous state as judged by SDS polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was estimated to be 31,000 by SDS-PAGE and 25,000 by gel filtration on TSKgel G2000SW. The optimum pH was 7.0 and the enzyme was stable at pH 7.0-9.0. The enzyme oxidized triphenols such as pyrogallol and phloroglucinol, and was completely inhibited by sulfide and cyanide, while it was neither affected by kojic acid nor N-hydroxyglycine (laccase inhibor). These results indicated that the enzyme had properties different from those of polyphenol oxidases from other sources such as mandarin orange and soybean.
- 社団法人日本農芸化学会の論文
- 1993-02-23
著者
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Oyama H
Kyoto Inst. Technol. Kyoto Jpn
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Oyama Hiroshi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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SHIN Takashi
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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Nomura Yoshiyuki
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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Tono Tetsuzo
Laboratory of Food Science, Faculty of Agriculture, Saga University
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Nomura Yoshiyuki
Department Of Applied Microbial Technology Faculty Of Engineering Sojo University
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Tono Tetsuzo
Laboratory Of Cellulose Chemistry
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Tono Tetsuzo
Laboratory Of Food Science Faculty Of Agriculture Saga University
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Shin T
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Shin Takashi
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Nomura Y
Kumamoto Inst. Technol. Kumamoto Jpn
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Takenisi Shigeyuki
Osaka Municipal Technical Research Institute
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SHIN Takashi
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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