Colorimetric Measurement of Total Activity of Leucine Aminopeptidase and Arylamidase in Serum Using Laccase-Catalyzed Oxidative Decarboxylation
スポンサーリンク
概要
- 論文の詳細を見る
A simple colorimetric method for measuring the total activity of leucine aminopeptidase (EC 3.4.11.1) and arylamidase (EC 3.4.11.2) in serum was developed. The assay method is based on the following series of reactions : leucine aminopeptidase and arylamidase hydrolyze a substrate, L-leucyl-L-2-(4-hydroxyphenyl)glycine, to liberate 2-(4-hydroxyphenyl)glycine. The compound is then converted into 4-hydroxybenzaldehyde by laccase-catalyzed oxidative decarboxylation. Finally, the 4-hydroxybenzaldehyde produced is colorimetrically measured by treatment with 2,4-dinitrophenylhydrazine to evaluate the total activity of these aminopeptidases.
- 社団法人日本生物工学会の論文
- 1994-05-25
著者
-
NAKASHIMA Takashi
Central Research Laboratories, Mercian Corp.
-
YOSHIOKA Takeo
Central Research Laboratories, Mercian Corp.
-
Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
-
Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
-
Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
-
SHIN Takashi
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
-
AGEMATU Hitosi
Central Research Laboratories, Mercian Corporation
-
Murao S
Sojo Univ. Kumamoto Jpn
-
Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Shin T
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
-
Shin Takashi
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
-
Agematu Hitosi
Central Research Laboratories Mercian Corporation
-
Yoshioka Takeo
Central Research Laboratories Mercian Corporation
-
Shibamoto Norio
Central Research Laboratories, Mercian Co.
-
Shibamoto Norio
Central Research Laboratories Mercian Co.
-
Agematu Hitosi
Central Research Laboratories Mercian Co.
-
Agematu H
Mercian Corp. Fujisawa Jpn
-
Takenisi Shigeyuki
Osaka Municipal Technical Research Institute
-
Nakashima Takashi
Central Research Laboratories Mercian Co.
-
MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
-
Yoshioka Takeo
Central Research Laboratories, Mercian Corporation
-
SHIN Takashi
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
関連論文
- Synthesis and Biological Evaluation of Cytogenin Derivatives
- Mer-f3,12-Hydroxy-ovalicin, Produced by Metarrhizium sp. f3
- Inhibition of Angiogenesis by a New Isocoumarin, NM-3
- Degradation of Streptomyces Metalloprotease Inhibitor (SMPI) by Neutral Protease from Bacillus subtilis var. amylosacchariticus
- Epoxyquinomicins A, B, C and D, New Antibiotics from Amycolatopsis II. Effect on Type II Collagen-induced Arthritis in Mice
- Acetoxydehydroaustin, a New Bioactive Compound, and Related Compound Neoaustin from Penicillium sp. MG-11
- Identification of the Microorganism Which Produces Epstein-Barr Virus Early Antigen-inducing Indole Alkaloids
- ACTIVE SITE STRUCTURE AND FUNCTION OF NEUTRAL PROTEASE FROM BACILLUS SUBTILIS var. AMYLOSACCHARITICUS
- Chemical Modification of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Tyrosyl Residues Iodinated(Biological Chemistry)
- Dye-sensitized Photooxidation of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Histidine Residue Oxidized(Biological Chemistry)
- Streptomyces Pepsin Inhibitor-insensitive Carboxyl Proteinase from Ganoderma lucidum
- Streptomyces Pepsin Inhibitor-Insensitive Carboxyl Proteinase from Lentinus edodes
- Purification and Some Properties of Carboxyl Proteinase in Mycelium of Lentinus edodes
- A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, and Identification of Catalytic Serine Residue
- Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
- A Novel Laccase Inhibitor, N-Hydroxyglycine, Produced by Penicillium citrinum YH-31
- PURIFICATION AND CHARACTERIZATION OF NEW ENZYMES, URETHANE HYDROLASES AND THEIR UTILITY
- New Enzymatic Colorimetric Analyses to Evaluate the Activities of Carboxypeptidase A and B(Analytical Chemistry)
- A New Screening Method for Microorganisms Which Produce Carboxypeptidases
- New Enzymatic Colorimetric Reactions of Benzoic Acid Derivatives with ABTS [2, 2-Azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] in the Presence of Laccase
- Purification and Some Properties of Urethane Hydrolase II from Lactobacillus casei ε ATCC 7469
- Isolation and Characterization of N^α-Benzyloxycarbonyl Amino Acid Urethane Hydrolase II from Lactobacillus fermenti 36 ATCC 9338(Biological Chemistry)
- Structures of the Laccase-catalyzed Oxidation Products of Hydroxy-benzoic Acids in the Presence of ABTS [2,2'-Azino-di-(3-ethylbenzothiazoline-6-sulfonic Acid)]
- A Simultaneous Assay Method for L-Glutamate and L-Pyroglutamate Contents in Soy Sauce Using a 5-Oxoprolinase (without ATP Hydrolyzing Activity)
- Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from Scytalidium lignicolum
- Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp. KU-22
- Isolation and Characterization of a Novel 5-Oxoprolinase (without ATP-hydrolyzing)from Alcaligenes faecalis N-38A
- A Novel Type of Proteinase, Masikinolysin, from Streptomyces griseoloalbus SN-22
- Isolation and Characterization of a Dipeptidyl Aminopeptidase from Streptomyces sp.WM-23
- Purification and Characterization of Arctium lappa L. (Edible Burdock) Polyphenol Oxidase
- Enhancement of Activities of Cellulases under High Hydrostatic Pressure
- Isolation and Identification of a Pyrogallol Oxidase Producing Microorganism and the Enzyme Production
- Localization of Glucomannan and Fucogalactomannan in Rhodotorula glutinis Cell Wall and Spheroplast Formation of Its Living Cell
- Purification and Properties of Three Xylanases from Aspergillus aculeatus
- Rhodopeptins(Mer-N1033), Novel Cyclic Tetrapeptides with Antifungal Activity from Rhodococcus sp. 1. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activities
- Mer-A2026A and B, Novel Piericidins with Vasodilating Effect II.Physico-Chemical Properties and Chemical Structures
- Mer-A2026A and B, Novel Piericidins with Vasodilating Effect I.Producing Organism, Fermentation, Isolation and Biological Properties
- Cloning and Characterization of pcd Encoding Δ'-Piperideine-6-Carboxylate Dehydrogenase from Flavobacterium lutescens IFO3084
- Characterization of L-Lysine 6-Aminotransferase and Its Structural Gene from Flavobacterium lutescens IFO3084
- Oxidative Decarboxylations of 4-Hydroxymandelic Acid and 2-(4-Hydroxyphenyl)glycine by Laccase from Coriolus versicolor and Bilirubin Oxidases from Trachyderma tsunodae and Myrothecium verrucaria
- Transformation of 7-(4-Hydroxyphenylacetamido)cephalosporanic Acid into a New Cephalosporin Antibiotic, 7-[1-Oxaspiro(2.5)octa-6-oxo-4, 7-diene-2-carboxamido]ceph-alosporanic Acid, by Laccase
- Coriolus versicolor Laccase Catalyzes the Decarboxylation of 2-(4-Hydroxyphenyl)-glycine and 4-Hydroxymandelic Acid
- Isolation and Characterization of a Proteinaceous Protease Inhibitor from Bacillus subtilis(Microbiology & Fermentation Industry)
- Additional Evidence for the Identity of Scytalidium lignicolum Acid Proteinases with the Carboxyl Proteinase Group : The Interaction between Angiotensin I and S-PI-Insensitive Acid Proteinases by Means of A Zinc(II)-Dye Complex as a Probe(Biological Chemi
- Characterization of the Complex between α_2-Macroglobulin and a Serine Proteinase from Bacillus natto(Biological Chemistry)
- Comparative Study on the Specificities of Several Fungal Aspartic and Acidic Proteinases towards the Tetradecapeptide of a Renin Substrate(Biological Chemistry)
- Carboxyl Proteinase Essential for Maturation of Basidiospores of Lentinus edodes (shiitake)
- Purification and Characterization of a Novel Type of Chitinase from Vibrio alginolyticus TK-22
- Purification and Characterization of an Alkaline Proteinase Produced by Pimelobacter sp. Z-483
- Isolation of Propioxatin A from Actinosynnema sp. SI-23 during a Screening for Serratia piscatorum Metalloproteinase Inhibitors
- Purification and Properties of an S-PI(Pepstatin Ac)-insensitive Carboxyl Proteinase from a Xanthomonas sp. Bacterium(Biological Chemistry)
- Stresgenin B, an Inhibitor of Heat-induced Heat Shock Protein Gene Expression, Produced by Streptomyces sp. AS-9
- Rhodopeptins, Novel Cyclic Tetrapeptides with Antifungal Activities from Rhodococcus sp. III. Synthetic Study of Rhodopeptins
- Rhodopeptins, Novel Cyclic Tetrapeptides with Antifungal Activities from Rhodococcus sp. II. Structure Elucidation
- Molecular Characterization of a Rhodotorula-lytic Enzyme from Paecilomyces lilacinus Having β-1, 3-Mannanase Activity
- Interaction of Proteinaceous Protease Inhibitor of Bacillus subtilis with Intracellular Proteases from the Same Strain(Microbiology & Fermentation Industry)
- Novel β-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from Acremonium sp. HI-25
- Properties of Ascorbate Oxidase Produced by Acremonium sp. HI-25
- Isolation and Purification of Ascorbate Oxidase from Acremonium sp.HI-25
- An Acidic Ascorbate Oxidase Produced by Acremonium sp. HI-25(Biological Chemistry)
- Inactivation of Scytatidium lignicolum Acid Protease B with 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propanet(Biological Chemistry)
- Different Action by Two Avicelases from Aspergillus aculeatus(Biological Chemistry)
- Reduced Cellulose as a Substrate of Cellulases(Biological Chemistry)
- Enzymatic Properties of Hydrocellulase from Aspergillus aculeatus
- A Novel Thermostable, S-PI(Pepstatin Ac)-insensitive Acid Proteinase from Thermophilic Bacillus novosp. Strain MN-32(Biological Chemistry)
- Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli
- Effects of α- and β-Arbutin on Activity of Tyrosinases from Mushroom and Mouse Melanoma
- Colorimetric Measurement of Total Activity of Leucine Aminopeptidase and Arylamidase in Serum Using Laccase-Catalyzed Oxidative Decarboxylation
- A New Microorganism Producing a Glucosyl Transfer Enzyme to Polyphenols
- Colorimetric Measurement of Angiotensin I-Converting Enzyme Activity Using Bilirubin Oxidase-Catalyzed Oxidative Decarboxylation
- Purification and Some Properties of a Thermostable Metal Proteinase Produced by Thermomicrobium sp. KN-22 Strain(Biological Chemistry)
- New Proteinacens α-Amylase inhibitor (T-76) from Streptomyces nitrosporeus
- Studies on β-Glucosidases from Soybeans That Hydrolyze Daidzin and Genistin : Isolation and Characterization of an Isozyme
- Enzymatic Properties of Two Avicelases from Aspergillus aculeatus No. F-50(Biological Chemistry)
- New Okaramine Congeners, Okaramines D, E, and F, from Penicillium simplicissimum ATCC 90288
- A Novel Pyrazine Compound Produced from Chitin by the Activity of the Enzyme from Vibrio alginolyticus TK-24
- Enzymatic Synthesis of (+)Catechin-α-glucoside and Its Effect on Tyrosinase Activity
- Molecular Cloning and Expression of Proteinaceous α-Amylase Inhibitor Gene from Streptomyces nitrosporeus in Escherichia coli
- Purification and Some Properties of a Haim-sensitive α-Amylase from Newly Isolated Bacillus sp. No. 195
- Okaramine C, a New Insecticidal Indole Alkaloid from Penicillium simplicissimum(Organic Chemistry)
- Identification of the Microorganism Which Produces Insecticidal Indole Alkaloids, Okaramines A and B(Microbiology & Fermentation Industry)
- Inhibition of Lysozyme Activity by Acidic Polymers(Biological Chemistry)
- Isolation of New Anthracyclines 10-O-Rhodosaminyl β-Rhodomycinone and β-Isorhodomycinone form Mild-acid Treated Culture of Obelmycin-producing Streptomyces violaceus
- New Betaclamycin and Aclarubicin Analogs Obtained by Prolonged Microbial Conversion with an Aclarubicin-negative Mutant
- Isolation and Characterization of a Novel Hen Egg White Lysozyme Inhibitor from Bacillus subtilis I-139(Biological Chemistry)
- Some Properties of Hen Egg White Lysozyme Inhibitor from Bacillus subtilis I-139(Biological Chemistry)
- Lysozyme Inhibitory Activity of DNA from Bacillus subtilis I-139(Biological Chemistry)
- Okaramine B, an Insecticidal Indole Alkaloid, Produced by Penicillium simplicissimum AK-40(Organic Chemistry)
- PA34 INSECTICIDAL INDOLE ALKALOIDS, OKARAMINE A AND B, PRODUCED BY PENICILLIUM SIMPLICISSIMUM
- Okaramine A, a Novel Indole Alkaloid with Insecticidal Activity, from Penicillium simplicissimum AK-40(Organic Chemistry)
- Isolation and Characterization of Fructokinase from Pseudomonas sp. KN-21
- Isolation and Characterization of a Serine Proteinase, Inactivating m-Subunit of Lactate Dehydrogenase, from Penicillium citrinum KE-1
- Purification and Some Properties of Trehalase from Chatomium aureum MS-27
- Reduction in the Time Required for Sterilization by Use of a Novel Sterilizer with Rotary Vibrators
- Identification of a Bacterium which Produced Cell Wall Lytic Enzyme for Red Yeast and Purification of the Enzyme : Studies on Red Yeast Cell Walls(V)
- A Novel Trehalase Inhibitor, Trehalostatin, Produced byAmycolatopsis trehalostatica HG-25(Microbiology & Fermentation Industry)
- Purification and Characterization of an α-Amylase Inhibitor (Haim) Produced by Streptomyces griseosporeus YM-25
- Inhibition of Sporulation of Bacillus subtilis by MAPI, a Serine Protease Inhibitor, and Interaction of MAPI with Membrane Bound Protease