Studies on β-Glucosidases from Soybeans That Hydrolyze Daidzin and Genistin : Isolation and Characterization of an Isozyme
スポンサーリンク
概要
- 論文の詳細を見る
β-Glucosidases (A, B, and C) have been isolated from soybean cotyledons. The isolated enzyme is a β-Glucosidase-C forming a single band on CM-Sephadex C-50 ion exchange chromatography, and on Butyl-Toyopearl 650C and Sephadex G-150 gel filtration. The homogeneity of the purified enzyme was proved by SDS-polyacrylamide gel electrophoresis and isoelectric focusing. The molecular weight was estimated to be 81,000 and 36,000 by HPLC gel filtration on TSKgel G3000SW_xL and SDS-polyacrylamide gel electrophoresis, respectively. The isoelectric point was estimated to be 6.68. The enzyme showed optimum activity at pH 4.5 and 45℃, and its pH range of action was 3.5-7.0. The enzyme was stable from pH 4.0to 6.0 at 5℃. The enzyme hydrolyzed the isoflavone glucosides, daidzin, and genistin, to yield daidzein and genistein .
- 社団法人日本農芸化学会の論文
- 1995-09-23
著者
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Matsuura Masaru
Research & Development Division, Kikkoman Corporation
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Matsuura Masaru
Research And Development Division Of Kikkoman Corporation
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SASAKI Jun
Research Center, Asahi Glass Co., Ltd.
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Suzuki J
Utsunomiya Univ. Utsunomiya Jpn
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Sasaki Jun
Research Center Asahi Glass Co. Ltd.
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Sasaki Jun
Research and Development Division of Kikkoman Corporation
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