Isolation and Characterization of a Novel Hen Egg White Lysozyme Inhibitor from Bacillus subtilis I-139(Biological Chemistry)
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概要
- 論文の詳細を見る
A novel lysozyme inhibitor, hen egg white lysozyme inhibitor (Hewli) was isolated from the culture broth of a bacterium (strain I-139) identified as Bacillus subtilis. Maximum lysozyme inhibitory activity was obtained when the bacterium was grown aerobically in a medium consisting of 0.65 % glucose, 0.5% sodium glutamate, 1 % meat extract, and 4% Polypepton (pH 7.0), at 30℃ after 30 to 35 hr. Hewli was purified 69-fold from the culture supernatant of B. subtilis I-139 by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, Sephacryl S-200 gel chromatography, DEAE-Toyopearl 650M column chromatography, affinity chromatography on a column of immobilized egg white lysozyme, and acetone extraction. The purified lysozyme inhibitor has an amino acid composition of aspartic acid, glutamic acid, valine, and leucine in a 1:1:1:4 molar ratio and fatty acid composition of palmitic acid and/or vaccenic acid. Hewli inhibited B. subtilis I-139 glycanase and animal lysozymes such as the hen, turkey, or human enzymes.
- 社団法人日本農芸化学会の論文
- 1990-05-23
著者
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Kato Masataka
Biwako Research Laboratory Otsuka Foods Co. Ltd.
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Wang San-Lang
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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Wang San-lang
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Wang San-lang
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Hoshino Masami
Biwako Research Laboratory Otsuka Foods Co. Ltd.
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Hoshino Masami
Biwako Research Institute Otsuka Foods Co. Ltd.
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