Cloning and Sequencing of an Exoglucanase Gene from Streptomyces sp. M23, and Its Expression in Streptomyces lividans TK-24(Genetics, Molecular Biology, and Gene Engineering)
スポンサーリンク
概要
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A gene encoding exoglucanase (CBHII) of Streptomyces sp. M23 was cloned and sequenced. The cbhII gene consisted of 1359bp capable of encoding a polypeptide of 453 amino acids with a calculated molecular mass of 45,175Da. The deduced amino acid sequence showed homology with those of cellulases belonging to family 6 of the glycosyl hydrolases. The cbhII gene was subcloned into the plasmid pSEV1 and expressed in Streptomyces lividans TK-24. The transformed cells were able to secrete the enzyme efficiently in an active form. The CBHII expressed by S. lividans TK-24 was purified to homogeneity by SDS-polyacrylamide gel and characterized. The recombinant CBHII was stable up to 50℃ and more than 30% of the original activity remained after heating at 100℃ for 10min. The amino-terminal amino acid sequence of the recombinant CBHII was identified as GPAAPTARVD. These results agreed well with the properties of the authentic CBHII.
- 社団法人日本生物工学会の論文
- 2005-04-25
著者
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Izumori K
Rare Sugar Research Center Kagawa University
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Izumori Ken
Rare Sugar Research Center Faculty Of Agriculture Kagawa University
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Izumori Ken
松谷化学工業 開発研究所
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Izumori Ken
Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University
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Takada G
Rare Sugar Research Center Kagawa University
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Takata Goro
Rare Sugar Res. Center And Fac. Of Agriculture Kagawa Univeristy
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Takada Goro
Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University
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Park Chang-su
Dep. Of Bioscience And Biotechnology Konkuk Univ. 1 Hwayang-dong Gwangjin-gu Seoul 143-701 Kor
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kawaguchi Takashi
Division of Cardiology, Kagawa Prefectural Shirotori Hospital
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Park Chang
Division of Cardiology, Department of Internal Medicine, Korea University Medical college
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Takada Goro
Rare Sugar Research Center, kagawa University
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Izumori Ken
Rare Sugar Res. Center Kagawa Univ.
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University
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Izumori Ken
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Takada Goro
秋田大学 医学部小児外科学
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SUMITANI JUNICHI
Division of Applied Biological Chemistry, Graduate School of Agriculture and Biological Sciences, Os
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ARAI MOTOO
Environmental Biology, College of Bioscience and Biotechnology, Chubu University
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Park Chang
Division Of Applied Biological Chemistry Graduate School Of Agriculture And Biological Sciences Osak
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Tajima Gou
Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences
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Takata Goro
Rare Sugar Research Center Kagawa University
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Sumitani J
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Kawaguchi Takashi
Division Of Cardiology Kagawa Prefectural Shirotori Hospital
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Park Chang-Su
Division of Applied Biological Chemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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Sumitani Jun-ichi
Division of Applied Biological Chemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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