Cloning and Transcription Analysis of the Aspergillus aculeatus No. F-50 Endoglucanase 2 (cmc2) Gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

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• The cmc2 gene, coding for an endoglucanase 2 (CMC2) of Aspergillus aculeatus, was cloned using both genomic and cDNA libraries, and sequenced. The gene consists of 1230 bp encoding a protein of 410 amino acid residues with a molecular mass of 43,697 Da. The CMC2, composed of an N-terminal catalytic domain belonging to the family 5 of glycosyl hydrolases and a C-terminal cellulose-binding domain (CBD) belonging to the family I of CBDs, showed identity with other fungal endoglucanases, particularly with that of A. niger, A. nidulans, A. kawachii and A. aculeatus. The transcription of the cmc2 gene in A. aculeatus cells that were grown on different carbon sources was measured. Analysis by the ribonuclease protection assay revealed that expression of the cmc2 gene is induced by cellulose and some disaccharides and repressed by glucose.

• 社団法人日本生物工学会の論文
• 2002-11-25

著者

• IZUMORI KEN

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  松谷化学工業 開発研究所

• Takada G

  Rare Sugar Research Center Kagawa University

• Takata Goro

  Rare Sugar Res. Center And Fac. Of Agriculture Kagawa Univeristy

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takada Goro

  秋田大学 医学部小児外科学

• KAWASAKI MASATOSHI

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• KITAWAKI MAKI

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• KAWAGUCHI TAKASHI

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• SUMITANI JUN-ICHI

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• ARAI MOTOO

  Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Kawaguchi Takashi

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Kawaguchi Takashi

  College Of Agriculture University Of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Drug Safety Research Eisai Co. Ltd.

• Arai M

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Arai Motoo

  Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University

• Kawaguchi T

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Kitawaki Maki

  Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University

• Takata Goro

  Rare Sugar Research Center Kagawa University

• Sumitani J

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Kawasaki Masatoshi

  Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University

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