Cloning and Characterization of the D-Tagatose 3-Epimerase Gene from Pseudomonas cichorii ST-24

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概要

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• The gene encoding D-tagatose 3-epimerase (D-TE) from Pseudomonas cichorii ST-24 was cloned and sequenced. It was found to consist of 873 bp encoding 290 amino acid residues. The molecular weight of the deduced amino acid sequence of D-TE was determined to be 32.5 kDa. The deduced amino acid sequence showed no extensive homology with sequences of other sugar-related epimerases, but homology was observed with several hypothetical proteins of procaryotes. i.e. Synechocystis sp., Bacillus subtilis, Haemophilus influenzae, and Escherichia coli. The D-TE gene was expressed in E. coli.

• 社団法人日本生物工学会の論文
• 1997-06-25

著者

• ITOH HIROMICHI

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• IZUMORI KEN

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  松谷化学工業 開発研究所

• Ishida Y

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University:department Of Biotechnolo

• KIMURA YOSHIO

  Department of Life Science, Faculty of Agriculture, Kagawa University

• ISHIDA YUTAKA

  Department of Food Science, Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Itoh Hiromichi

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Ishida Yuzaburo

  Laboratory Of Microbiology; Department Of Fisheries Faculty Of Agriculture Kyoto University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Ishida Yutaka

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kagawa University

• KAMIYA TAKANORI

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• Kamiya Takanori

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Kimura Yoshio

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

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