Purification and Characterization of L-Fucose Dehydrogenase from Agrobacterium tumefaciens K-28
スポンサーリンク
概要
- 論文の詳細を見る
A bacterium that produces L-fucose dehydrogenase [EC 1.1.1.122] constitutively was isolated from soil, and identified as Agrobacterium tumefaciens K-28. The enzyme, which was extracted from cells grown in a medium containing 1% sucrose as a sole carbon source, was purified about 150-fold with a 51.6% yield, and was found to be homogeneous on polyacrylamide gel electrophoresis (PAGE). The molecular weight of the purified enzyme was about 100,000 and the pI was about 4.4. The optimum pH was at 9.0 to 10.0,with pH stability from 7.5 to 9.0.
- 1994-01-25
著者
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ITOH HIROMICHI
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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IZUMORI KEN
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Izumori K
Rare Sugar Research Center Kagawa University
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Izumori Ken
Rare Sugar Research Center Faculty Of Agriculture Kagawa University
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Izumori Ken
松谷化学工業 開発研究所
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University
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Izumori Ken
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Itoh H
Osaka City Inst. Public Health And Environmental Sci. Osaka Jpn
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Itoh Hiromichi
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka
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Yoshino A
Nec Corp. Kanagawa Jpn
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Yoshino Akira
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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MIYOSHI TERUMI
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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SHIRAGAMI KEIKO
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Miyoshi Terumi
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Shiragami Keiko
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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