D-Allose Production from D-Psicose Using Immobilized L-Rhamnose Isomerase
スポンサーリンク
概要
- 論文の詳細を見る
D-Psicose was produced from D-fructose using an immobilized D-tagatose 3-epimerase(D-TE), and used as a substrate for the production of D-allose by immobilized L-rhamnose isomerase(L-RI). D-Allose was produced from D-psicose by L-RI, which was constitutively produced by a mutant strain of Pseudomonas sp. LL172 and immobilized on chitopearl beads of BCW 2603. The isomerization reaction progressed steadily while the concentration of D-psicose was increased from 2.5 to 20% in Tris-HCl buffer(0.05M. pH7.5), and about 40% of D-psicose was converted to D-allose in all the cases. The immobilized enzyme was stable even after repeated use over 20d at 40℃ in the preparation of D-allose without detectable decrease in the enzyme activity, indicating the high stability of the enzyme in the batch reaction. The crystallized product was confirmed as D-allose by analysis of the IR spectra, NMR spectra, melting point and optical rotation.
- 公益社団法人日本生物工学会の論文
- 1998-05-25
著者
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Bhuiyan Shakhawat
Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University
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ITAMI YOSHIYUKI
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka
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Itami Yoshiyuki
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Shakhawat Hossain
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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KATAYAMA TAKESHI
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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ROKUI YUKO
Department of Bioresource science, Faculty of Agriculture, Kagawa University
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Rokui Yuko
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Katayama Takeshi
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Katayama Takeshi
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University
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