Preparation of D-Sorbose from D-Tagatose by Immobilized D-Tagatose 3-Epimerase
スポンサーリンク
概要
- 論文の詳細を見る
D-Tagatose 3-epimerase (D-TE) from Pseudomonas sp. ST-24 was immobilized on various types of Chitopearl beads. The highest activity was found in D-TE immobilized on Chitopearl beads of BCW 2503,the yield being about 80% of free enzyme applied. Maximum activity of the immobilized enzyme was obtained at pH 7-9 and around 60℃. The enzyme was stable in a pH range of 7-10,and below 60℃. In a high concentration (30%) of substrate, the reaction progressed without substrate inhibition. Two grams of D-sorbose crystals could be obtained from 3 g D-tagatose. Furthermore, in a batch reaction repeated five times, about 70% of D-tagatose was converted to D-sorbose each time.
- 社団法人日本生物工学会の論文
- 1995-02-25
著者
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Khan Anisur
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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ITOH HIROMICHI
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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SATO TOMOKO
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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TAKEUCHI TOMOHIRO
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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IZUMORI KEN
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Khan A
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Izumori K
Rare Sugar Research Center Kagawa University
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Izumori Ken
Rare Sugar Research Center Faculty Of Agriculture Kagawa University
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Izumori Ken
松谷化学工業 開発研究所
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University
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Izumori Ken
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Itoh H
Osaka City Inst. Public Health And Environmental Sci. Osaka Jpn
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Itoh Hiromichi
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka
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Sato Takeshi
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Takeuchi Tomohiro
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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