Production of D-Lyxose from D-Glucose by Microbial and Enzymatic Reactions

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概要

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• D-Arabitol was first prepared from D-glucose using Candida famata R28. The reaction gave 5.0% D-arabitol from 10.0% D-glucose. D-Arabitol was then almost completely converted to D-xylulose using Acetobacter aceti IFO 3281. Finally, D-lyxose was prepared from D-xylulose enzymatically usng L-ribose isomerase from toluene-treated cells of Acinetobacter sp.strain DL-28. The isomerization reaction progressed steadily and the concentration of D-xylulose increased from 1.0 to 10.0%. About 70% of D-xylulose was converted to D-lyxose in all cases. Separation of residual D-xylulose from the reaction mixture is very difficult to achieve by column chromatography, but D-xylulose could be selectively degraded easily using Saccharomyces cerevisiae IFO 0841. The product was crystallized and was confirmed to be D-lyxose by HPLC, ^<13>C-NMR spectra, IR spectra analysis, and optical rotation measurement.

• 社団法人日本生物工学会の論文
• 1999-12-25

著者

• IZUMORI KEN

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Bhuiyan S

  Kagawa Univ. Kagawa Jpn

• Bhuiyan Shakhawat

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  松谷化学工業 開発研究所

• Ahmed Zakaria

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Takada G

  Rare Sugar Research Center Kagawa University

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• SASAHARA HIROYUKI

  Food Research Institute, Kagawa Prefectural Government

• SAIKI TETSUYA

  Department of Biochemistry and Food Sciences, Faculty of Agriculture, Kagawa University

• SHIMONISHI TSUYOSHI

  Department of Biochemistry and Food Sciences, Faculty of Agriculture, Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Bhuiyan Shakhawat

  Depatrment Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Saiki Tetsuya

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Takata Goro

  Rare Sugar Research Center Kagawa University

• Shimonishi T

  National Food Res. Inst. Ibaraki Jpn

• Sasahara H

  Kagawa Prefectural Government Kagawa‐ken Jpn

• Sasahara Hiroyuki

  Food Research Institute Kagawa Prefectural Covernment

• Bhuiyan Sakhawat

  Department of Biochemistry and Food Sciences, Faculty of Agriculture, Kagawa University

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