A Novel Bioconversion of L-Fructose to L-Glucose by Klebsiella pneumoniae

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概要

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• L-Fructose, which was produced from L-psicose using immobilized D-tagatose 3-epimerase, was utilized as a starting material in the preparation of an uncommon aldose-hexose, L-glucose, by cell reaction. A mutant strain, Klebsiella pneumoniae strain 40bXX, produced D-arabinose isomerase constitutively. Toluene-treated cells of the mutant strain, which were used as the source of crude D-arabinose isomerase, were employed in the conversion of L-fructose to L-glucose. Empirically, 0.35 g of L-glucose was obtained from 1.Og of L-fructose, viz an overall yield of 35%. The product obtained was purified and identified to be L-glucose by high performance liquid chromatography (HPLC) analysis, and was ultimately confirmed by ^<13>C nuclear magnetic resonance (^<13>C NMR) spectra.

• 社団法人日本生物工学会の論文
• 2003-03-25

著者

• IZUMORI KEN

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  Dep. Of Bioresource Sci. Fac. Of Agriculture Kagawa Univ.

• Izumori Ken

  松谷化学工業 開発研究所

• Leang Khim

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Takada G

  Rare Sugar Research Center Kagawa University

• Takata Goro

  Rare Sugar Res. Center And Fac. Of Agriculture Kagawa Univeristy

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• SULTANA ISHRAT

  Department of Biochemistry and Food Science,Faculty of Agriculture,Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Sultana Ishrat

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Takada Goro

  秋田大学 医学部小児外科学

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Takata Goro

  Rare Sugar Research Center Kagawa University

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