Cloning, Nucleotide Sequence, and Transcriptional Analysis of Aspergillus aculeatus No.F-50 Cellobiohydrolase I(cbhI)Gene

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概要

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• The cbhl gene, coding for a major cellobiohydrolase(CBHI)of Aspergillus aculeatus, was cloned and sequenccd. The gene consists of 1620-bp and encodes a protein containing 540 amino acids with a calculated molecular mass of 56, 123 Da. CBHI, composed of an N-terminal catalytic domain belonging to family 7 of the glycosyl hydrolages, and a C-terminal cellulose-binding domain(CBD)belonging to family I of the CBDs, showed high similarity with other fungal CBHIs, especially with that of Penicillium janthinellum. The cbhI gene transcription start points in A. aculeatus were defined by primer extension, and the putative promoter sequence was analyzed. This sequence was found to be closely related to the consensus sequences of various fungal genes. Transcription analysis by ribonuclease protection assay revealed that the cbhI gene is induced by lowmolecular-weight cellooligosaccharide and repressed by glucose. The results emphasize the possibility that in the A. aculeatus cellulase system, cellobiose is the true inducer and the role of the cbhI gene lies within the cascade regulating cellulase induction.

• 社団法人日本生物工学会の論文
• 1998-01-25

著者

• KAWAGUCHI Takashi

  Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Takada G

  Rare Sugar Research Center Kagawa University

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Arai Motoo

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Kawaguchi Takashi

  College Of Agriculture University Of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Drug Safety Research Eisai Co. Ltd.

• Arai M

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Arai Motoo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Kawaguchi T

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• SUMITANl JUN-ICHI

  Department of Applied Biological Chemistry, Faculty of Agriculture, University of Osaka Prefecture

• Takata Goro

  Rare Sugar Research Center Kagawa University

• Sumitani J

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Sumitani Jun-ichi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

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