Expression of the Chitinase III Gene of Aeromonas sp. No. 10S-24 in Escherichia coli
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概要
- 論文の詳細を見る
The chitinase III gene of Aeromonas sp. No. 10S-24 was expressed in Escherichia coli. Production of chitinase III by E. coli was 3-fold higher than that of chitinases in the culture supernatant of Aeromonas sp. The enzyme from E. coli was purified and characterized. The molecular weight of the chitinase III from E. coli was estimated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) to be 55,000. This agreed with the calculated molecular weight of the mature chitinase III (54,111). However, it was different from that of the enzyme from Aeromonas sp. It showed that the chitinase III from Aeromonas sp. had an additional sugar chain, causing the higher molecular weight. Chitinase III from E. coli had almost the same enzymatic properties as chitinase III from Aeromonas sp., but the specific activity of the chitinase III from E. coli is slightly higher than that of the native chitinase III. Both enzymes hydrolyzed chitosan (80% deacetylated) well.
- 社団法人日本農芸化学会の論文
- 1996-07-23
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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UEDA MITSUHIRO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Ueda M
Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive
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Ueda Mitsuhiro
Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Sciences Osaka Prefectu
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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SHIRO Minoru
Department of Applied Biochemistry, College of Agriculture, Osaka Prefecture University
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Shiro Minoru
Department Of Applied Biochemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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Ueda Mitsuhiro
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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