Expression of a Gene Encoding Chitinase(pCA 8 ORF)from Aeromonas sp.No.10S-24 in Escherichia coli and Enzyme Characterization

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概要

• 論文の詳細を見る

• A gene encoding chitinase from Aeromonas sp.no.10S-24 was expressed using pTrc99A in Escherichia coli JM 105 which yielded a 5-fold higher activity than when pUC19 was used. Three different truncated enzymes(SA-1, SA-2 and SA-3)were obtained after purification. Their isoelectric points were 7.0, 6.9, and 6.7, respectively. The enzymes showed two optimum pHs, 4.0 and 7.0, when incubated with ethylene glycol chitin as the substrate, and were stable over a wide pH range(3.0-9.0). The optimum temperature was 60℃ and the enzymes were stable up to 50℃. The chitinases exhibited wide substrate specificities for chitin-related compounds.

• 社団法人日本生物工学会の論文
• 2001-06-25

著者

• KAWAGUCHI Takashi

  Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring

• INUI Hiroshi

  Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osak

• NAKANO Yoshihisa

  Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osak

• Sutrisno Aji

  阪府大院・生命環境科学

• Sutrisno Aji

  Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive

• Sutrisno Aji

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Nakano Y

  Dep. Of Applied Biological Chemistry Graduate School Of Life And Environmental Sciences Osaka Prefec

• Miyatake K

  Osaka Prefecture Univ. Osaka Jpn

• Inui H

  Center For Res. And Dev. Of Bioresources Osaka Prefecture Univ.

• Inui H

  Tottori Univ. Tottori Jpn

• Inui Hiroshi

  Department Of Agricultural Biochemistry And Biotechnology Tottori University:(present Address)depart

• Inui Hiroshi

  Department Of Applied Biological Chemistry Osaka Prefecture University

• Inui Hiroshi

  Department Of Agricultural Biochemistry And Biotechnology Tottori University

• Inui Hiroshi

  Graduate School Of Life And Environmental Sciences Of Osaka Prefecture University

• SUTRISNO Aji

  阪府大院農・応生化

• NAKANO Yoshihisa

  Graduate School of Life and Environmental Sciences of Osaka Prefecture University

• Arai Motoo

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• MIYATAKE Kazutaka

  Department of Applied Biological Chemistry, Osaka Prefecture University

• MATSUDA Kazuhiko

  Laboratory of Plant Pathology and Biotechnology and Laboratory of Pesticide Chemistry

• UEDA MITSUHIRO

  Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University

• Nakano Yoshihisa

  Graduate School Of Life And Environmental Science Osaka Prefecture University

• Nakano Yoshihisa

  Department Of Agriculture University Of Osaka Prefecture

• Nakano Yoshihisa

  Nutrition Laboratory College Of Agriculture

• Kawaguchi Takashi

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Kawaguchi Takashi

  College Of Agriculture University Of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Drug Safety Research Eisai Co. Ltd.

• 切畑 光統

  大阪府立大学 農学部

• Kirihata Mitsunori

  Graduate School Of Life And Environmental Science Osaka Prefecture University

• Arai M

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Arai Motoo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Ueda M

  Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive

• Ueda Mitsuhiro

  Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Sciences Osaka Prefectu

• Miyatake Kazutaka

  Department Of Agricultural Chemistry University Of Osaka Prefecture

• Kawaguchi T

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Nakano Yoshihisa

  Department Of Agricultural Chemistry

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

• Ueda Mitsuhiro

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

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